Tree Genetics & Genomes

, Volume 1, Issue 3, pp 109–115

Analysis of 70,000 EST sequences to study divergence between two closely related Populus species

  • Per Unneberg
  • Michael Strömberg
  • Joakim Lundeberg
  • Stefan Jansson
  • Fredrik Sterky
Original Paper

DOI: 10.1007/s11295-005-0014-0

Cite this article as:
Unneberg, P., Strömberg, M., Lundeberg, J. et al. Tree Genetics & Genomes (2005) 1: 109. doi:10.1007/s11295-005-0014-0
  • 66 Downloads

Abstract

The Populus genus has evolved as the model organism for forest tree genomics, which has been further emphasised with the sequencing of the Populus trichocarpa genome. Populus species are widely spread over the Northern Hemisphere and provide a great source of genetic diversity, which can be used for mapping of quantitative trait loci, positional cloning, association mapping and studies in environmental adaptation. Collections of expressed sequence tags (ESTs) are rich sources in studies of genetic diversity. Here, we report on an in-depth analysis of 70,000 ESTs from two Populus species, Populus tremula and Populus trichocarpa. We present data on the level of conservation in transcript sequences and supply a collection of potential single nucleotide polymorphisms.

Keywords

EST SNP Assembly cDNA library analysis Ortholog 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Per Unneberg
    • 1
  • Michael Strömberg
    • 1
  • Joakim Lundeberg
    • 1
  • Stefan Jansson
    • 2
  • Fredrik Sterky
    • 1
  1. 1.Department of BiotechnologyAlbaNova University Center, Royal Institute of TechnologyStockholmSweden
  2. 2.Umeå Plant Science Center, Department of Plant PhysiologyUmeå UniversityUmeåSweden

Personalised recommendations