Contamination issues in a continuous ethanol production corn wet milling facility

  • Esha Khullar
  • Angela D. Kent
  • Timothy D. Leathers
  • Kenneth M. Bischoff
  • Kent D. Rausch
  • M. E. Tumbleson
  • Vijay Singh
Original Paper

DOI: 10.1007/s11274-012-1244-6

Cite this article as:
Khullar, E., Kent, A.D., Leathers, T.D. et al. World J Microbiol Biotechnol (2013) 29: 891. doi:10.1007/s11274-012-1244-6

Abstract

Low ethanol yields and poor yeast viability were investigated at a continuous ethanol production corn wet milling facility. Using starch slurries and recycle streams from a commercial ethanol facility, laboratory hydrolysates were prepared by reproducing starch liquefaction and saccharification steps in the laboratory. Fermentations with hydrolysates prepared in the laboratory were compared with plant hydrolysates for final ethanol concentrations and total yeast counts. Fermentation controls were prepared using hydrolysates (plant and laboratory) that were not inoculated with yeast. Hydrolysates prepared in the laboratory resulted in higher final ethanol concentrations (15.8 % v/v) than plant hydrolysate (13.4 % v/v). Uninoculated controls resulted in ethanol production from both laboratory (12.2 % v/v) and plant hydrolysates (13.7 % v/v), indicating the presence of a contaminating microorganism. Yeast colony counts on cycloheximide and virginiamycin plates confirmed the presence of a contaminant. DNA sequencing and fingerprinting studies also indicated a number of dissimilar communities in samples obtained from fermentors, coolers, saccharification tanks, and thin stillage.

Keywords

ContaminationWet millingEthanol

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Esha Khullar
    • 1
  • Angela D. Kent
    • 2
  • Timothy D. Leathers
    • 3
  • Kenneth M. Bischoff
    • 3
  • Kent D. Rausch
    • 1
  • M. E. Tumbleson
    • 1
  • Vijay Singh
    • 1
  1. 1.Agricultural and Biological EngineeringUniversity of IllinoisUrbanaUSA
  2. 2.Department of Natural Resources and Environmental SciencesUniversity of IllinoisUrbanaUSA
  3. 3.National Center for Agricultural Utilization Research, ARS, USDAPeoriaUSA