World Journal of Microbiology and Biotechnology

, Volume 27, Issue 12, pp 2759–2766

Optimization of media composition and culture conditions for acarbose production by Actinoplanes utahensis ZJB-08196

  • Ya-Jun Wang
  • Li-Ling Liu
  • Zhi-Hua Feng
  • Zhi-Qiang Liu
  • Yu-Guo Zheng
Original Paper

DOI: 10.1007/s11274-011-0751-1

Cite this article as:
Wang, YJ., Liu, LL., Feng, ZH. et al. World J Microbiol Biotechnol (2011) 27: 2759. doi:10.1007/s11274-011-0751-1

Abstract

Acarbose is a clinically useful drug for the treatment of type II, insulin-independent diabetes as a hypoglycemic agent. An acarbose-overproducing strain ZJB-08196, indentified as Actinoplanes utahensis, was able to produce 4,210 mg l−1 of acarbose at 591 mOsm kg−1 with the optimized conditions at bench scale. Shake flask fermentation showed that maltose, glycerol and monosodium glutamate were supportive for acarbose production; soybean meal had higher bioavailability than corn steep liquor. Moreover, acarbose formation was not parallel with mycelial growth and the pattern of acarbose production by A. utahensis ZJB-08196 was the type of mixed-growth associated.

Keywords

AcarboseActinoplanes utahensisIdentificationFermentationGlycerol

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Ya-Jun Wang
    • 1
  • Li-Ling Liu
    • 1
  • Zhi-Hua Feng
    • 1
  • Zhi-Qiang Liu
    • 1
  • Yu-Guo Zheng
    • 1
  1. 1.Institute of BioengineeringZhejiang University of TechnologyHangzhou City, Zhejiang ProvincePeople’s Republic of China