World Journal of Microbiology and Biotechnology

, Volume 27, Issue 8, pp 1755–1764

Chlorothalonil degradation by Ochrobactrum lupini strain TP-D1 and identification of its metabolites

  • Xiu-Zhen Shi
  • Rong-Jun Guo
  • Kazuhiro Takagi
  • Zuo-Qing Miao
  • Shi-Dong Li
Original Paper

DOI: 10.1007/s11274-010-0631-0

Cite this article as:
Shi, XZ., Guo, RJ., Takagi, K. et al. World J Microbiol Biotechnol (2011) 27: 1755. doi:10.1007/s11274-010-0631-0

Abstract

Chlorothalonil (2, 4, 5, 6-tetrachloroisophthalonitrile, TPN) has been widely used as a wide-spectrum fungicide in China and other countries, and is considered to be an important soil and water contaminant. Here we report the isolation and characterization of a novel TPN-degrading bacterial strain TP-D1 from a heavily TPN-polluted soil in Henan Province, China, and identified it as a strain of Ochrobactrumlupini based on 16S rRNA gene sequence analysis and its morphological, biochemical, and physiological characteristics. Strain TP-D1 could degrade 90.4 and 99.7% of TPN after 4- and 7-day incubation in mineral salt broth with 50 mg TPN l−1 and in autoclaved soil with 50 μg TPN g−1, respectively. Two new metabolites, methyl 2, 5, 6-trichloro-3-cyano-4-methoxy-benzoate (metabolite C) and methyl 3-cyano-2, 4, 5, 6-tetrachlorobenzoate (metabolite D), were detected besides previously reported 4-hydroxy-2, 5, 6-trichloroisophthalonitrile (TPN-OH, metabolite A). This result suggests that the cyano-group in TPN could be converted into amide groups by strain TP-D1, and reveal the biodegradation mechanism of TPN in soil.

Keywords

Chlorothalonil (TPN) Metabolic pathway Ochrobactrum lupini Biodegradation 

Abbreviations

BHC

Hexachlorcyclonhexane

CCHT

1-Carbamoyl-3-cyano-4-hydroxy-2, 5, 6-trichlorobenzene

c.f.u.

Colony forming units

CTB

3-Cyano-2, 4, 5, 6-tetrachlorobenzamide

DDT

Dichloro-diphenyl-trichloroethane

DTTC

1, 3-Dicarbamoyl-2, 4, 5, 6-tetrachlorobenzene

ECD

Electron capture detector

GC

Gas chromatography

HPLC

High performance liquid chromatography

LB

Luria-Bertani

LC-ESI-MS

Liquid chromatography-electrospray ionization mass spectrometry

LC-APCI-MS

Liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

MS

Mineral salt

NA

Nutrient agar

PCNB

Pentachloronitrobenzene

SPE

Solid-phase extraction

TPN

Chlorothalonil; 2, 4, 5, 6-tetrachloroisophthalonitrile

TPN-OH

4-Hydroxy-2, 5, 6-trichloroisophthalonitrile

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Xiu-Zhen Shi
    • 1
  • Rong-Jun Guo
    • 1
  • Kazuhiro Takagi
    • 2
  • Zuo-Qing Miao
    • 1
  • Shi-Dong Li
    • 1
  1. 1.Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.National Institute for Agro-Environmental SciencesTsukuba, IbarakiJapan

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