Original Paper

World Journal of Microbiology and Biotechnology

, Volume 27, Issue 3, pp 495-503

First online:

Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

  • Xu Yu ChenAffiliated withHainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeHainan Provincial Key Laboratory of Resources, Conservation and Development of Southern Medicine
  • , Yao Dong QiAffiliated withInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Chinese Peking Union Medical College
  • , Jian He WeiAffiliated withHainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeHainan Provincial Key Laboratory of Resources, Conservation and Development of Southern Medicine Email author 
  • , Zheng ZhangAffiliated withInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Chinese Peking Union Medical College
  • , De Li WangAffiliated withHainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeHainan Provincial Key Laboratory of Resources, Conservation and Development of Southern Medicine
  • , Jin Dong FengAffiliated withHainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeHainan Provincial Key Laboratory of Resources, Conservation and Development of Southern Medicine
  • , Bing Chun GanAffiliated withHainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical CollegeHainan Provincial Key Laboratory of Resources, Conservation and Development of Southern Medicine

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Abstract

Fifty-two endophytic fungi strains with different colony morphologies were isolated from stems, leaves and roots of Huperzia serrata (Thunb. ex Murray) Trevis. collected from Bawangling Reserve of Hainan Province in southern China. They were identified mainly based on rDNA ITS sequences and phylogenetic analysis. The results showed that all strains belonged to four classes, i.e. Sordariomycetes (92.31%), Dothideomycetes (3.85%), Pezizomycetes (1.92%) and Agaricomycetes (1.92%). Forty-seven strains were identified at the genus level, including Glomerella (Colletotrichum), Hypocrea (Trichoderma), Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus. As to the other five strains, three were identified at the order level and two at the family level, indicating that a great diversity of fungi taxa exists in H. serrata. Most isolated strains belonged to the genus of Glomerella (Colletotrichum) and Hypoxylon, twenty-one from Glomerella and its anamorph Colletotrichum (42.3% of total isolated strains) and ten from Hypoxylon (19.2% of total isolated strains). Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus were reported as endophytic fungi isolated from H. serrata for the first time.

Keywords

Huperzia serrata Endophytic fungi Identification Phylogenetic analysis