Coinoculation of Bacillus thuringeinsis-KR1 with Rhizobium leguminosarum enhances plant growth and nodulation of pea (Pisum sativum L.) and lentil (Lens culinaris L.)

  • Pankaj K. Mishra
  • Smita Mishra
  • G. Selvakumar
  • J. K. Bisht
  • Samresh Kundu
  • Hari Shankar Gupta
Original Article

DOI: 10.1007/s11274-009-9963-z

Cite this article as:
Mishra, P.K., Mishra, S., Selvakumar, G. et al. World J Microbiol Biotechnol (2009) 25: 753. doi:10.1007/s11274-009-9963-z

Abstract

Nodulation and the subsequent nitrogen fixation are important factors that determine the productivity of legumes. The beneficial effects of nodulation can be enhanced when rhizobial inoculation is combined with plant-growth-promoting bacteria (PGPB). The PGPB strain Bacillusthuringiensis-KR1, originally isolated from the nodules of Kudzu vine (Puerariathunbergiana), was found to promote plant growth of field pea (Pisumsativum L.) and lentil (Lensculinaris L.) under Jensen’s tube, growth pouch and non-sterile soil, respectively, when co-inoculated with Rhizobiumleguminosarum-PR1. Coinoculation with B. thuringiensis-KR1 (at a cell density of 106 c.f.u. ml−1) provided the highest and most consistent increase in nodule number, shoot weight, root weight, and total biomass, over rhizobial inoculation alone. The enhancement in nodulation due to coinoculation was 84.6 and 73.3% in pea and lentil respectively compared to R. leguminosarum-PR1 treatment alone. The shoot dry-weight gains on coinoculation with variable cell populations of B. thuringiensis-KR1 varied from 1.04 to 1.15 times and 1.03 to 1.06 times in pea and lentil respectively, while root dry weight ratios of coinoculated treatments varied from 0.98 to 1.14 times and 1.08 to 1.33 times in pea and lentil respectively, those of R. leguminosarum-PR1 inoculated treatment at 42 days of plant growth. While cell densities higher than 106 c.f.u. ml−1 had an inhibitory effect on nodulation and plant growth, lower inoculum levels resulted in decreased cell recovery and plant growth performance. The results of this study indicate the potential of harnessing endophytic bacteria of wild legumes for improving the nodulation and growth of cultivated legumes.

Keywords

Bacillusthuringiensis-KR1 Endophytic bacteria Lensculinaris L. Pisumsativum L. Plant growth promotion Rhizobiumleguminosarum-PR1 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Pankaj K. Mishra
    • 1
  • Smita Mishra
    • 1
  • G. Selvakumar
    • 1
  • J. K. Bisht
    • 1
  • Samresh Kundu
    • 2
  • Hari Shankar Gupta
    • 1
  1. 1.Vivekananda Institute of Hill Agriculture, (Indian Council of Agricultural Research)AlmoraIndia
  2. 2.I.I.S.S., (Indian Council of Agricultural Research)BhopalIndia

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