World Journal of Microbiology and Biotechnology

, 26:171

Prevalence of multidrug-resistant Staphylococcus aureus in diabetics clinical samples

  • S. Raju
  • Ajay Kumar Oli
  • S. A. Patil
  • R. Kelmani Chandrakanth
Original Paper

DOI: 10.1007/s11274-009-0157-5

Cite this article as:
Raju, S., Oli, A.K., Patil, S.A. et al. World J Microbiol Biotechnol (2010) 26: 171. doi:10.1007/s11274-009-0157-5

Abstract

Antibiotic resistance in 40 Staphylococcus aureus clinical isolates from 110 diabetic patients (36%) was evaluated. Of these, 32 (80%) of the isolates showed multidrug-resistance to more than eight antibiotics and 35% isolates were found to be methicillin resistant S. aureus (MRSA). All 40 S. aureus strains (100%) screened from diabetic clinical specimens were resistant to penicillin, 63% to ampicillin, 55% to streptomycin, 50% to tetracycline and 50% to gentamicin. Where as low resistance rate was observed to ciprofloxacin (20%) and rifampicin (8%). In contrast, all (100%) S. aureus strains recorded susceptibility to teicoplanin, which was followed by vancomycin (95%). Genotypical examination revealed that 80% of the aminoglycoside resistant S. aureus (ARSA) have aminoglycoside modifying enzyme (AME) coding genes; however, 20% of ARSA which showed non-AME mediated (adaptive) aminoglycoside resistance lacked these genes in their genome. In contrast all MRSA isolates possessed mecA, femA genetic determinants in their genome.

Keywords

Methicillin resistant Staphylococcus aureusAminoglycoside modifying enzymesmecA and femA

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • S. Raju
    • 1
    • 3
  • Ajay Kumar Oli
    • 1
  • S. A. Patil
    • 2
  • R. Kelmani Chandrakanth
    • 1
  1. 1.Department of BiotechnologyGulbarga UniversityGulbargaIndia
  2. 2.Department of NeuromicrobiologyNational Institute of Mental Health and Neuro SciencesBangaloreIndia
  3. 3.Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia