Original Paper

World Journal of Microbiology and Biotechnology

, Volume 25, Issue 8, pp 1471-1477

Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates

  • Mohammad Ali MalboobiAffiliated withApplied Microbiology Research Group, Jahad Daneshgahi, Tehran UnitNational Institute of Genetic Engineering and Biotechnology Email author 
  • , Parviz OwliaAffiliated withDepartment of Microbiology, Shahed University
  • , Mandana BehbahaniAffiliated withDepartment of Biotechnology, Faculty of Agriculture, Bu-Ali-Sina University
  • , Elaheh SarokhaniAffiliated withApplied Microbiology Research Group, Jahad Daneshgahi, Tehran Unit
  • , Sara MoradiAffiliated withApplied Microbiology Research Group, Jahad Daneshgahi, Tehran Unit
  • , Bagher YakhchaliAffiliated withNational Institute of Genetic Engineering and Biotechnology
  • , Ali DeljouAffiliated withDepartment of Biotechnology, Faculty of Agriculture, Bu-Ali-Sina University
  • , Kambiz Morabbi HeraviAffiliated withNational Institute of Genetic Engineering and Biotechnology

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Abstract

Screening soil samples collected from a diverse range of slightly alkaline soil types, we have isolated 22 competent phosphate solubilizing bacteria (PSB). Three isolates identified as Pantoea agglomerans strain P5, Microbacterium laevaniformans strain P7 and Pseudomonas putida strain P13 hydrolyzed inorganic and organic phosphate compounds effectively. Bacterial growth rates and phosphate solubilization activities were measured quantitatively under various environmental conditions. In general, a close association was evident between phosphate solubilizing ability and growth rate which is an indicator of active metabolism. All three PSB were able to withstand temperature as high as 42°C, high concentration of NaCl upto 5% and a wide range of initial pH from 5 to 11 while hydrolyzing phosphate compounds actively. Such criteria make these isolates superior candidates for biofertilizers that are capable of utilizing both organic and mineral phosphate substrates to release absorbable phosphate ion for plants.

Keywords

Phosphate solubilization Pantoea agglomerans Microbacterium laevaniformans and Pseudomonas putida Biofertilizer