Direct visualization of bacterial infection process in nematode hosts by an improved immunocytochemical method
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- Tian, BY., Ke, CR., Huang, W. et al. World J Microbiol Biotechnol (2009) 25: 909. doi:10.1007/s11274-008-9945-6
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Our previous work on Brevibacillus laterosporus G4 had demonstrated that an extracellular protease BLG4 played a key role as a pathogenic factor in bacterial infection of nematodes. In this study, we developed an improved method for the immunofluorescent localization of protease BLG4 to directly visualize the bacterial infection process in a nematode host and demonstrate the involvement of the protease in penetrating nematode cuticles in vivo. This work supplies a new basic knowledge that elucidates the microbial infection processes in nematodes.