Direct visualization of bacterial infection process in nematode hosts by an improved immunocytochemical method

  • Bao-Yu Tian
  • Chong-Rong Ke
  • Wei Huang
  • Ke-Qin Zhang
  • Jian-Zhong Huang
Short Communication

DOI: 10.1007/s11274-008-9945-6

Cite this article as:
Tian, BY., Ke, CR., Huang, W. et al. World J Microbiol Biotechnol (2009) 25: 909. doi:10.1007/s11274-008-9945-6

Abstract

Our previous work on Brevibacillus laterosporus G4 had demonstrated that an extracellular protease BLG4 played a key role as a pathogenic factor in bacterial infection of nematodes. In this study, we developed an improved method for the immunofluorescent localization of protease BLG4 to directly visualize the bacterial infection process in a nematode host and demonstrate the involvement of the protease in penetrating nematode cuticles in vivo. This work supplies a new basic knowledge that elucidates the microbial infection processes in nematodes.

Keywords

Brevibacillus laterosporus Infection Nematode Immuno-labeled fluorescence Biocontrol 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Bao-Yu Tian
    • 1
  • Chong-Rong Ke
    • 1
  • Wei Huang
    • 1
  • Ke-Qin Zhang
    • 2
  • Jian-Zhong Huang
    • 1
  1. 1.Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life SciencesFujian Normal UniversityFuzhouPeople’s Republic of China
  2. 2.Laboratory for Conservation and Utilization of Bio-resourcesYunnan UniversityKunmingPeople’s Republic of China

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