World Journal of Microbiology and Biotechnology

, 25:601

Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: prescreening, ribotyping and metabolic diversity

  • Imran Sajid
  • Clarisse Blandine Fotso Fondja Yao
  • Khaled Attia Shaaban
  • Shahida Hasnain
  • Hartmut Laatsch
Original Paper

DOI: 10.1007/s11274-008-9928-7

Cite this article as:
Sajid, I., Fotso Fondja Yao, C.B., Shaaban, K.A. et al. World J Microbiol Biotechnol (2009) 25: 601. doi:10.1007/s11274-008-9928-7

Abstract

A culture collection of 110 indigenous Streptomyces strains originally isolated from saline farmlands (Punjab, Pakistan) using stringent methods was screened biologically and chemically to investigate their potential for the production of bioactive secondary metabolites. In a biological screening the crude extracts obtained from the culture broth of selected strains were analysed for their activity against a set of test organisms, including Gram-positive, Gram-negative bacteria, fungi and microalgae using the disk diffusion bioassay method. Additionally a cytotoxicity test was performed by means of the brine shrimp microwell cytotoxicity assay. In a chemical screening each of the crude extracts was analysed by TLC using various staining reagents and by HPLC-MS/MS measurements. The results depicted an impressive chemical diversity of crude extracts produced by these strains. The taxonomic status of the selected strains was confirmed by preliminary physiological testing and 16S rRNA gene sequencing.

Keywords

Indigenous Streptomyces sppPrescreeningRibotypingMetabolite diversityHPLC-MS/MS

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Imran Sajid
    • 1
    • 2
  • Clarisse Blandine Fotso Fondja Yao
    • 2
  • Khaled Attia Shaaban
    • 2
  • Shahida Hasnain
    • 1
  • Hartmut Laatsch
    • 2
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of the PunjabLahorePakistan
  2. 2.Institute of Organic and Biomolecular Chemistry, University of GöttingenGottingenGermany