Original Paper

World Journal of Microbiology and Biotechnology

, Volume 25, Issue 3, pp 383-390

First online:

Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

  • Jing-Wei LinAffiliated withSchool of Life Sciences, Northeast Normal University
  • , Li-Xia HaoAffiliated withSchool of Life Sciences, Northeast Normal University
  • , Gui-Xue XuAffiliated withSchool of Life Sciences, Northeast Normal University
  • , Fei SunAffiliated withInstitute of Frontier Medical Science of Jilin University
  • , Feng GaoAffiliated withMedical Center, Duke University
  • , Ren ZhangAffiliated withDepartment of Biological Sciences, University of Wollongong
  • , Li-Xia LiuAffiliated withSchool of Life Sciences, Northeast Normal University Email author 

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The fungal immunomodulatory proteins (FIPs) are a new protein family identified from several edible and medical mushrooms and play an important role in anti-tumor, anti-allergy and immunomodulating activities. A gene encoding the FIP was cloned from the mycelia of Changbai Lingzhi (Ganoderma lucidum) and recombinant expressed in the Pichia pastoris expression system. SDS-PAGE, amino acid composition and circular dichroism analyses of the recombinant FIP (reFIP) indicated that the gene was correctly and successfully expressed. In vitro assays of biological activities revealed that the reFIP exhibited similar immunomodulating capacities as native FIPs. The reFIP significantly stimulated the proliferation of mouse spleen lymphocytes and apparently enhanced the expression level of interleukin-2 released from the mouse splenocytes. In addition, anti-tumor activity assay showed that the reFIP could inhibit the proliferation of human leukemia-NB4 by inducing the cell apoptosis to a degree of about 32.4%. Taken together, the FIP gene from Changbai G. lucidum has been integrated into the yeast genome and expressed effectively at a high level (about 191.2 mg l−1). The reFIP possessed very similar biological activities to native FIPs, suggesting its potential application as a food supplement or immunomodulating agent in pharmaceuticals and even medical studies.


Fungal immunomodulatory protein Ganoderma lucidum Molecular cloning Recombinant expression Pichia pastoris