World Journal of Microbiology and Biotechnology

, Volume 24, Issue 10, pp 2171–2178

A DNA vaccine candidate for B. anthracis immunization, pcDNA3.1+PA plasmid, induce Th1/Th2 mixed responses and protection in mice

  • Fatemeh Vahedi
  • Jennelle Kyd
  • Gholamreza Moazeni Jula
  • Monavvar Afzalaghaeei
  • Mahdi Kianizadeh
  • Mahmoud Mahmoudi
Original Paper

DOI: 10.1007/s11274-008-9725-3

Cite this article as:
Vahedi, F., Kyd, J., Moazeni Jula, G. et al. World J Microbiol Biotechnol (2008) 24: 2171. doi:10.1007/s11274-008-9725-3

Abstract

The protective antigen (PA) of Bacillus anthracis (B. anthracis) is a potent immunogen and a candidate subunit vaccine. To address the question whether antibodies raised against PA following injection of pcDNA3.1+PA plasmid, encoding PA, can protect against virulent B. anthracis two different regimens of PA based vaccines (DNA and live spore) were used. The groups of BALB/c mice that received live spores of the Sterne strain, naked pcDNA3.1 and naked pcDNA3.1+PA were compared to control groups. All groups were injected three times with 30-day intervals. Two weeks after the last immunization, all mice were subjected to challenge with a pathogenic strain of B.anthracis (C2). Blood samples were taken before each injection and challenge. Evaluation of the sera by ELISA method showed that DNA immunization using pcDNA3.1+PA plasmid resulted in an antibody profile representative of a mixed Th1 and Th2 response, with a skewing to a Th1 response. The group which received the naked pcDNA3.1+PA had a survival rate of >80%. This challenge assay revealed that antibodies raised following DNA vaccination against PA can confer strong protection, and resistance against virulent species of B. anthracis.

Keywords

DNA vaccineBacillus anthracispcDNA3.1

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Fatemeh Vahedi
    • 1
  • Jennelle Kyd
    • 2
  • Gholamreza Moazeni Jula
    • 3
  • Monavvar Afzalaghaeei
    • 4
  • Mahdi Kianizadeh
    • 1
  • Mahmoud Mahmoudi
    • 5
  1. 1.Biotechnology DepartmentRazi Vaccine & Serum Research InstituteMashhadIran
  2. 2.Capricornia Center of Mucosal ImmunologyCentral Queensland UniversityRock HamptonAustralia
  3. 3.Department of Aerobic Bacterial Vaccine ProductionRazi Vaccine & Serum Research InstituteKarajIran
  4. 4.Department of Social MedicineMashhad University of Medical ScienceMashhadIran
  5. 5.Immunology Research CenterMashhad University of Medical ScienceMashhadIran