World Journal of Microbiology and Biotechnology

, Volume 23, Issue 12, pp 1747–1754

Isolation and characterization of novel Serratia marcescens (AY927692) for pentachlorophenol degradation from pulp and paper mill waste

  • Shail Singh
  • R. Chandra
  • D. K. Patel
  • Vibhuti Rai
Original Paper

DOI: 10.1007/s11274-007-9424-5

Cite this article as:
Singh, S., Chandra, R., Patel, D.K. et al. World J Microbiol Biotechnol (2007) 23: 1747. doi:10.1007/s11274-007-9424-5

Abstract

Seven aerobic bacterial strains were isolated from pulp paper mill waste and screened for pentachlorophenol (PCP) tolerance on PCP containing mineral salt agar medium (MSM). The organism was characterized by 16S rDNA sequencing which showed 99.7% sequence similarity with Serratiamarcescens. PCP degradation was routinely monitored with spectrophotometric analysis and further confirmed by HPLC analysis. Among seven strains, ITRC S7 was found to degrade up to 90.33% of 1.127 mM (300 mg/l) of PCP and simultaneous release of chloride ion (2.435 mM) emphasized the bacterial dechlorination in the medium in presence of glucose as an additional carbon and energy source under optimized condition within 168 h incubation. In absence of glucose bacterium was unable to utilize PCP indicating the phenomenon of co-metabolism. Bacterium was identified as S. marcescens (AY927692), was a novel and potential aerobic bacterial strain capable of degrading PCP in axenic condition. Further, this strain may be used for bioremediation of PCP containing pulp paper mill waste in the environment.

Keywords

Dechlorination Degradation Pentachlorophenol Serratia marcescens 16S rDNA 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Shail Singh
    • 1
  • R. Chandra
    • 1
  • D. K. Patel
    • 2
  • Vibhuti Rai
    • 3
  1. 1.Environmental Microbiology SectionIndustrial Toxicology Research CentreLucknowIndia
  2. 2.Analytical Chemistry SectionIndustrial Toxicology Research CentreLucknowIndia
  3. 3.School of Life SciencesPandit Ravi Shankar Shukla UniversityRaipurIndia