World Journal of Microbiology and Biotechnology

, Volume 23, Issue 3, pp 443–446

Recombinant production of Penicillium oxalicum PJ3 phytase in Pichia Pastoris

  • Jaecheon Lee
  • Yunjaie Choi
  • Peter-Changwhan Lee
  • Seungha Kang
  • Jinduck Bok
  • Jaiesoon Cho
Brief Communication

DOI: 10.1007/s11274-006-9236-z

Cite this article as:
Lee, J., Choi, Y., Lee, PC. et al. World J Microbiol Biotechnol (2007) 23: 443. doi:10.1007/s11274-006-9236-z

Abstract

The 1,332 bp phytase gene of Penicilliumoxalicum PJ3 was inserted into the expression vector, pPICZαA and expressed in the methylotrophic yeast, Pichia pastoris as an active, extracellular phytase. The recombinant phytase reached a maximum yield of 12 U/ml of medium at 120 h of cultivation after methanol induction under shake-flask conditions. The enzyme was glycosylated, with a molecular mass of about 62.5 kDa. The Michaelis constant (Km) and maximum reaction rate (Vmax) for sodium phytate was 0.37 mM and 526.3 U/mg of protein, respectively. The optimal activity occurred at pH 4.5 and 55°C.

Keywords

Penicillium oxalicum Phytase Phytate Pichia pastoris Recombinant 

Abbreviations

YNB:

yeast nitrogen base with ammonium sulfate and without amino acids

YPD:

yeast extract peptone dextrose

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Jaecheon Lee
    • 1
  • Yunjaie Choi
    • 1
  • Peter-Changwhan Lee
    • 1
  • Seungha Kang
    • 1
  • Jinduck Bok
    • 3
  • Jaiesoon Cho
    • 2
  1. 1.School of Agricultural Biotechnology, College of Agriculture and Life SciencesSeoul National UniversitySeoul Korea
  2. 2.Inositol Signaling Group, LST, National Institute of Environmental Health Sciences, NIH, DHSSDurhamUSA
  3. 3.Choong Ang Biotech Co., Ltd.Ansan-CityKorea