World Journal of Microbiology and Biotechnology

, Volume 23, Issue 3, pp 375–382

Keratinolytic proteases of Bacillus species isolated from the Amazon basin showing remarkable de-hairing activity

  • Janice Luhering Giongo
  • Françoise S. Lucas
  • Franciani Casarin
  • Philipp Heeb
  • Adriano Brandelli
Original Paper

DOI: 10.1007/s11274-006-9234-1

Cite this article as:
Giongo, J.L., Lucas, F.S., Casarin, F. et al. World J Microbiol Biotechnol (2007) 23: 375. doi:10.1007/s11274-006-9234-1

Abstract

Three keratinolytic Bacillus spp. isolated from the Brazilian Amazon basin were characterized. The strains P6, P7 and P11 were identified based on morphological and biochemical characteristics and 16S rDNA sequences. P6, P7 and P11 sequences shared more than 99% similarity with B. subtilis, B. amyloliquefaciens and B. velesensis. The keratinases produced by these bacteria were active on azokeratin and degradation of feather barbules was observed. The enzymes were inhibited by the serine protease inhibitor PMSF, and showed maximum activity at pH 9.0. Proteins like albumin, casein and gelatin were hydrolysed by these keratinases. Depilatory studies on bovine pelts revealed that all three strains were efficient in promoting de-hairing. Microscopic analysis showed that the epidermis was completely removed and the absence of hair in follicles was observed.

Keywords

BacillusProteaseKeratinaseFeatherLeatherDepilatory activity

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Janice Luhering Giongo
    • 1
  • Françoise S. Lucas
    • 2
    • 3
  • Franciani Casarin
    • 1
  • Philipp Heeb
    • 2
    • 4
  • Adriano Brandelli
    • 1
  1. 1.Laboratório de Bioquímica e Microbiologia Aplicada, ICTAUniversidade Federal do Rio Grande do SulPorto AlegreBrasil
  2. 2.Department of Ecology and EvolutionUniversity of LausanneLausanneSwitzerland
  3. 3.Cereve, UPVM-Faculté des Sciences et TechnologiesCréteil CedexFrance
  4. 4.Laboratoire Evolution et Diversité BiologiqueUniversité Paul Sabatier IIIToulouse, Cedex 9France