Optimization of Xylanase Production by Thermomyces Lanuginosus in Tomato Seed Meal Using Response Surface Methodology

  • Petros Katapodis
  • Vasiliki Christakopoulou
  • Paul Christakopoulos
Original paper

DOI: 10.1007/s11274-005-9063-7

Cite this article as:
Katapodis, P., Christakopoulou, V. & Christakopoulos, P. World J Microbiol Biotechnol (2006) 22: 501. doi:10.1007/s11274-005-9063-7

Summary

A 32 central composite experimental design was performed with the aim of optimizing xylanase production by Thermomyces lanuginosus grown on corn cobs in submerged cultures. Xylanase production was first tested on different nitrogen sources (tomato skin, tomato seed meal, corn steep liquor, meat peptone, bacto-tryptone and yeast extract). Tomato seed meal was the selected substrate to test the effect of two variables on xylanase production (corn cobs and tomato seed meal concentrations). A second-order quadratic model and a response surface method showed that the optimum condition for xylanase production was corn cobs 4.6% (w/v) and tomato seed meal 2.1% (w/v). The optimum conditions found were transferred to 7-l bioreactors, where activities as high as 1630 U/ml were obtained.

Keywords

Response surface method Thermomyces lanuginosus tomato seed meal waste utilization xylanase 

Copyright information

© Springer 2006

Authors and Affiliations

  • Petros Katapodis
    • 1
  • Vasiliki Christakopoulou
    • 1
  • Paul Christakopoulos
    • 1
  1. 1.Biotechnology Laboratory, Chemical Engineering DepartmentNational Technical University of AthensAthensGreece