Keratinase Production by Newly Isolated Antarctic Actinomycete Strains Article DOI:
Cite this article as: Gushterova, A., Vasileva-Tonkova, E., Dimova, E. et al. World J Microbiol Biotechnol (2005) 21: 831. doi:10.1007/s11274-004-2241-1 Summary
The ability of actinomycete strains newly isolated from Antarctic soils to produce keratinolytic enzymes during growth on sheep wool waste was investigated. The strains which displayed highest keratinase activity and identified as
Streptomyces flavis 2BG (mesophilic) and Microbispora aerata IMBAS-11A (thermophilic) were selected for a more detailed analysis. The addition of starch to the growth medium affected keratinase secretion by both strains. After 5 days of cultivation, a 6-fold increase in keratinase activity of strain 11A was observed in the presence of 11 g starch/l and a 9-fold increase in keratinase activity of the strain 2BG in the presence of 5 g starch/l. The results obtained showed that both newly isolated strains are very promising for effective processing of native keratinous wastes. To our knowledge, this is the first report of Antarctic actinomycete strains that were able to grow on keratin-containing wastes by producing keratinolytic enzymes. Keywords Antarctic actinomycetes keratinase activity Microbispora aerata Streptomyces flavis wool waste Download to read the full article text References
Agre, N.S. 1986 Taxonomy of thermophilic actinomycetes. Ph.D. Thesis, Puschino, Moscow, pp. 5–10 (in Russian).
Atlas of actinomycetes. 1997 ed. Miyadoh, S. Asakura, Japan: Society for Actinomycetes. ISBN4-254-17098-X.
Bockle, B., Galunsky, B., Muller, R. 1995 Characterization of a keratinolytic serine proteinase from
Streptomyces pactum DSM 40530 Applied and Environmental Microbiology 61 3705 3710 Google Scholar Bockle, B., Muller, R. 1997 Reduction of disulphide bonds by Streptomyces pactum during growth on chicken feathers Applied and Environmental Microbiology 63 790 792 Google Scholar Cheng, S.W., Hu, H.M., Shen, S.W., Takagi, H., Asano, A., Tsai, Y.C. 1995 Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-1 Bioscience, Biotechnology and Biochemistry 59 2239 2243 CrossRef Google Scholar Ellman, G.L. 1959 Tissue sulfhydryl groups Archives of Biochemistry and Biophysics 82 70 77 CrossRef Google Scholar
Encyclopedia Britannica 2004 Antarctica. In
Encyclopedia Britannica, from Deluxe Edition 2004 CD-ROM. Gessesse, A., Hatti-Kaul, R., Gashe, B.A., Mattiasson, B. 2003 Novel alkaline proteases from alkaliphilic bacteria grown on chicken feather Enzyme and Microbial Technology 32 519 524 CrossRef Google Scholar Gradisar, H., Kern, S., Friedrich, J. 2000 Keratinase of Doratomyces microsporus Applied Microbiology and Biotechnology 53 196 200 Google Scholar
Gushterova, A., Noustorova, M., Tzvetkova, R., Spassov, G. & Chipeva, V. 1999
Investigations of the microflora in penguin’s excrements in the Antarctic. Vol. 2, pp. 1–7. Bulgarian Antarctic Research, Life Sciences, Sofia-Moscow: Pensoft Publishers. Gushterova, A., Ivanova, V., Aleksieva, K., Kolarova, M., Schlegel, B., Graefe, U. 2003 Taxonomic investigations of the Antarctic mesophilic strain 2BG, producer of secondary metabolites Biotechnology and Biotechnological Equipment 18 72 76 Google Scholar Ignatova, Z., Gousterova, A., Spassov, G., Nedkov, P. 1998 Decomposition of native keratin by thermophilic actinomycetes Comptes Rendus de l’Academie Bulgare des Sciences 51 67 70 Google Scholar Ignatova, Z., Gousterova, A., Spassov, G., Nedkov, P. 1999 Isolation and partial characterization of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus Canadian Journal of Microbiology 45 217 222 CrossRef Google Scholar Ivanova, V., Graefe, U., Schlegel, R., Schlegel, B., Gousterova, A., Kolarova, M., Aleksieva, K. 2003 Isolation and structure elucidation of tyramine and indole alkaloids from Antarctic strain Microbispora aerata IMBAS-11A Biotechnology and Biotechnological Equipment 17 128 133 Google Scholar Kabadjova, P., Vlahov, S., Dalgalarrondo, M., Dousset, X., Haertle, T., Briand, L., Chobert, J.-M. 1996 Isolation and distribution of Streptomyces populations with heat-resistant collagenlytic activity from two protein-rich areas in Bulgarian soils Folia Microbiologica 41 423 429 Google Scholar Kosmachev, A.E. 1954Thermophilic actinomycetes and their antagonistic properties Inst. Mikrobiol. Moscow Google Scholar Kunert, J. 1989 Biochemical mechanism of keratin degradation by the actinomycete Streptomyces fradiae and the fungus Microsporum gypseum: a comparison Journal of Basic Microbiology 29 597 604 Google Scholar Kunert, J., Stransky, Z. 1988 Thiosulfate production from cystine by keratinolytic prokaryote Streptomyces fradiae Archives of Microbiology 150 600 601 CrossRef Google Scholar Kunert, J., Truper, H.G. 1986 Cystine catabolism in mycelia of Microsporum gypseum, a dermatophytic fungus Archives of Microbiology 145 181 186 CrossRef Google Scholar Malviya, H.K., Rajak, R.C., Hasija, S.K. 1992 Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatine factory in Jabalpur Mycopathology 120 1 4 CrossRef Google Scholar
Noustorova, M., Gushterova, A., Tzvetkova, R. & Chipeva, V. 1999 Investigations of the microflora in glaciers from the Antarctic. Vol. 2. pp. 8–12. Bulgarian Antarctic Research, Life Sciences, Sofia-Moscow: Pensoft Publishers.
Onifade, A.A., Al-Sane, N.A., Al-Mussallam, A.A., Al-Zarbam, S. 1998 Potential for biotechnological application of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources Bioresource Technology 66 1 11 CrossRef Google Scholar Ryckeboer, J., Mergaert, J., Coosemans, J., Deprins, K., Swings, J. 2003 Microbiological aspects of biowaste during composting in monitored compost bin Journal of Applied Microbiology 94 127 137 CrossRef Google Scholar Santos, R.M.D.B., Firmino, A.A.P., De Sa, C.M., Felix, C.R. 1996 Keratinolytic activity of Aspergillus fumigatus Fresenius Current Microbiology 33 364 370 CrossRef Google Scholar Wang, J.-J., Shih, J.C.H. 1999 Fermentation production of keratinase from Bacillus licheniformis PWD-1 and B. subtilis FDB-29 Journal of Industrial Microbiology and Biotechnology 22 608 616 CrossRef Google Scholar