Article

Water, Air, & Soil Pollution

, Volume 223, Issue 7, pp 4141-4152

First online:

Nitrate Reductase-Dependent Nitric Oxide Production Is Involved in Microcystin-LR-Induced Oxidative Stress in Brassica rapa

  • Jian ChenAffiliated withInstitute of Food Quality and Safety, Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Safety Monitoring and Management of Ministry of AgricultureKey Laboratory of Food Quality and Safety of Jiangsu Province (State Key Laboratory Breeding Base)
  • , You Ming ZhongAffiliated withInstitute of Food Quality and Safety, Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Safety Monitoring and Management of Ministry of AgricultureKey Laboratory of Food Quality and Safety of Jiangsu Province (State Key Laboratory Breeding Base)
  • , Hai Qiang ZhangAffiliated withInstitute of Food Quality and Safety, Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Safety Monitoring and Management of Ministry of AgricultureKey Laboratory of Food Quality and Safety of Jiangsu Province (State Key Laboratory Breeding Base)College of Life Sciences, Nanjing Agricultural University
  • , Zhi Qi ShiAffiliated withInstitute of Food Quality and Safety, Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Safety Monitoring and Management of Ministry of AgricultureKey Laboratory of Food Quality and Safety of Jiangsu Province (State Key Laboratory Breeding Base) Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Histochemical and biochemical approaches were used to investigate the phytotoxicity induced by microcystin-LR (MC-LR) in the shoots of Brassica rapa seedlings. MC-LR exposure was able to induce oxidative stress by triggering the over-generation of reactive oxygen species (ROS) including superoxide anion radical (O2 ) and hydrogen peroxide (H2O2) in the shoots of B. rapa. MC-LR exposure led to the significant increase in the concentration of endogenous nitric oxide (NO) in B. rapa. However, such increase was completely suppressed by the treatment with nitrate reductase (NR) inhibitor NaN3, while l-NMMA, a NO synthase (NOS) inhibitor, had only slight effect on the content of endogenous NO in MC-LR-treated plant. These data suggested that NR-dependent pathway was the main source for endogenous NO generation under MC-LR stress. Afterwards, treatment with NaN3 reduced the ROS generation, lipid peroxidation, and loss of membrane integrity in MC-LR-treated plant. MC-LR stress induced the increase in the expression of superoxide dismutase, ascorbate peroxidase, and catalase. However, such an effect could be reversed by the treatment with NaN3. These results indicate that NR-dependent NO production mediates MC-LR-induced oxidative stress by triggering the over-generation of ROS in B. rapa.

Keywords

Brassica rapa Microcystin-LR Nitrate reductase Nitric oxide Oxidative stress