Water, Air, and Soil Pollution

, 204:19

Optimization of Culture Conditions for the Biodegradation of Lindane by the Polypore Fungus Ganoderma australe

  • V. Dritsa
  • F. Rigas
  • D. Doulia
  • E. J. Avramides
  • I. Hatzianestis
Article

DOI: 10.1007/s11270-009-0022-z

Cite this article as:
Dritsa, V., Rigas, F., Doulia, D. et al. Water Air Soil Pollut (2009) 204: 19. doi:10.1007/s11270-009-0022-z

Abstract

The bracket-like polypore fungus, Ganoderma australe, was selected for its potential to degrade lindane in liquid agitated sterile cultures. An orthogonal central composite design based on response surface methodology was used to find the optimum biodegradation and biosorption conditions of this pesticide and the growth conditions of the fungus. The factors tested include nitrogen content, initial concentration of lindane, incubation time, and temperature. The optimization parameters investigated were fungus biomass, fungus growth rate, final pH, specific biodegradation, specific biosorption, specific biodegradation rate, biodegraded to biosorbed ratio. The results of the experiments were statistically analyzed and the significance and effect of each factor on responses was assessed. The optimum (maximum) lindane biodegradation (3.11 mg biodegraded lindane per gram biomass) was obtained with nitrogen content of 1.28 g/L, lindane concentration of 7.0 ppm, temperature of 18.0°C, and 5 days of cultivation time.

Keywords

BiodegradationLindaneCentral composite design (CCD)Response surface methodology (RSM)Ganoderma australe

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • V. Dritsa
    • 1
  • F. Rigas
    • 1
  • D. Doulia
    • 1
  • E. J. Avramides
    • 2
  • I. Hatzianestis
    • 3
  1. 1.School of Chemical EngineeringNational Technical University of AthensAthensGreece
  2. 2.National Agricultural Research FoundationAthens Soil Science InstituteAthensGreece
  3. 3.National Center for Marine ResearchAthensGreece