Virus Genes

, Volume 48, Issue 1, pp 81–88

Characterization of a complete genome of a circular single-stranded DNA virus from porcine stools in Korea

  • A Reum Kim
  • Hee Chun Chung
  • Hye Kwon Kim
  • Eun Ok Kim
  • Van Giap Nguyen
  • Min Gyung Choi
  • Hye Jung Yang
  • Jung Ah Kim
  • Bong Kyun Park
Article

DOI: 10.1007/s11262-013-1003-2

Cite this article as:
Kim, A.R., Chung, H.C., Kim, H.K. et al. Virus Genes (2014) 48: 81. doi:10.1007/s11262-013-1003-2

Abstract

Porcine circular single-stranded DNA viruses have been just identified from swine feces in Korea. This virus was mentioned as bovine stool-associated circular DNA virus (BoSCV)-like virus discovered from porcine stools. However, the thorough characteristics of the virus were not identified. Therefore, this research focuses on finding a full genome sequence and analyzing the genetic features of the virus. The virus, now called porcine stool-associated circular DNA virus in Korea (PoSCV Kor), consists of 2,589 bases forming circular structure. It has two major ORFs inversely encoding replicase and capsid protein, with each stem–loop structure between 5′ ends and 3′ ends of the two putative ORFs. This characteristics is the same as PoSCV in New Zealand, but different from chimpanzee stool-associated circular virus (ChiSCVs) and BoSCV, which have one stem–loop structure. Therefore, it would be sure that PoSCV Kor is very similar to PoSCV in respect to the genetic aspect; the same number of nucleotide bases and the amino acid identity of replicase and capsid protein (96 and 93 %, respectively). This fact could be certified through the finding that PoSCV Kor and PoSCV are in the same cluster by phylogenetic analysis based on the comparison with full-sequences of other circular ssDNA viruses.

Keywords

PoSCVKoreaGenetic analysisCircular DNA virusComplete genome

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • A Reum Kim
    • 1
  • Hee Chun Chung
    • 1
  • Hye Kwon Kim
    • 2
  • Eun Ok Kim
    • 1
  • Van Giap Nguyen
    • 1
  • Min Gyung Choi
    • 1
  • Hye Jung Yang
    • 1
  • Jung Ah Kim
    • 1
  • Bong Kyun Park
    • 1
  1. 1.Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary ScienceSeoul National UniversitySeoulKorea
  2. 2.Research Evaluation TeamInstitute for Basic ScienceDaejeonKorea