, Volume 47, Issue 3, pp 408-413
Date: 14 Aug 2013

HIV-1 genotypic diversity and prevalence of drug resistance among treatment naïve HIV-infected individuals in Chengdu of China

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Abstract

AIDS is becoming one of leading cause of death among infectious diseases in China. There has been rapidly increasing epidemic of HIV/AIDS in Chengdu since 2003. In order to investigate HIV-1 subtype distribution, drug resistance-associated mutations as well as drug resistance prevalence among treatment naïve HIV-infected individuals in Chengdu of China, 244 HIV confirmed-reactive serum samples were collected from 2007 to 2010, including 165 obtained from blood centers and 79 obtained from hospitals. HIV-1 pol including whole protease, and partial reverse transcriptase genes was amplified, sequenced, and analyzed for subtype determination and drug resistance profile description. A total of 159 amplified sequences which were acquired from 98 sample obtained at blood centers and 61 samples obtained at hospitals had the following genotype distribution: G (0.6 %), F1 (0.6 %), A1 (0.6 %), B (1.9 %), circulating recombinant form (CRF) 01_AE (42.8 %), CRF07_BC (49.1 %), and CRF08_BC (4.4 %). There were 12.2 and 24.6 % samples obtained from blood centers and hospitals, respectively, that harbored drug resistance-associated mutations, and the prevalence of drug resistance among all cases was 1.3 %. This was the first report of HIV molecular surveillance among treatment naïve HIV-infected individuals in Chengdu. Our findings were believed to be helpful on contribution to comprehensive HIV control program in China.

Peibin Zeng and Yu Liu have contributed equally to the work.