Article

Virus Genes

, Volume 45, Issue 3, pp 610-613

Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus

  • Ricardo SalvadorAffiliated withInstituto de Microbiología y Zoología Agrícola (IMYZA-CICVyA-INTA)Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET Email author 
  • , M. Leticia FerrelliAffiliated withInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET
  • , Marcelo F. BerrettaAffiliated withInstituto de Microbiología y Zoología Agrícola (IMYZA-CICVyA-INTA)
  • , Wataru MitsuhashiAffiliated withNational Institute of Agrobiological Sciences
  • , Marina E. BiedmaAffiliated withInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICETINSERM U748, Institut de Virologie, Faculté de Médecine, Université de Strasbourg
  • , Víctor RomanowskiAffiliated withInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET
  • , Alicia Sciocco-CapAffiliated withInstituto de Microbiología y Zoología Agrícola (IMYZA-CICVyA-INTA)

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Abstract

The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.

Keywords

GP37 Baculovirus EpapGV Granulovirus Entomopoxvirus