Virus Genes

, Volume 45, Issue 3, pp 610–613

Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus

  • Ricardo Salvador
  • M. Leticia Ferrelli
  • Marcelo F. Berretta
  • Wataru Mitsuhashi
  • Marina E. Biedma
  • Víctor Romanowski
  • Alicia Sciocco-Cap
Article

DOI: 10.1007/s11262-012-0800-3

Cite this article as:
Salvador, R., Ferrelli, M.L., Berretta, M.F. et al. Virus Genes (2012) 45: 610. doi:10.1007/s11262-012-0800-3

Abstract

The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.

Keywords

GP37 Baculovirus EpapGV Granulovirus Entomopoxvirus 

Supplementary material

11262_2012_800_MOESM1_ESM.eps (2.6 mb)
Supplementary material 1: Comparison of the predicted amino acid sequences (EPS 2687 kb)
11262_2012_800_MOESM2_ESM.eps (1.4 mb)
Supplementary material 2: Table with accession numbers (EPS 1435 kb)
11262_2012_800_MOESM3_ESM.eps (1 mb)
Supplementary material 3: Comparison of relative location of chitinase and gp37/CBP genes (EPS 1068 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ricardo Salvador
    • 1
    • 2
  • M. Leticia Ferrelli
    • 2
  • Marcelo F. Berretta
    • 1
  • Wataru Mitsuhashi
    • 3
  • Marina E. Biedma
    • 2
    • 4
  • Víctor Romanowski
    • 2
  • Alicia Sciocco-Cap
    • 1
  1. 1.Instituto de Microbiología y Zoología Agrícola (IMYZA-CICVyA-INTA)CastelarArgentina
  2. 2.Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias ExactasUniversidad Nacional de La Plata, CONICETLa PlataArgentina
  3. 3.National Institute of Agrobiological SciencesTsukubaJapan
  4. 4.INSERM U748, Institut de Virologie, Faculté de Médecine, Université de StrasbourgStrasbourgFrance