Virus Genes

, Volume 44, Issue 2, pp 262–272

Molecular detection of novel picornaviruses in chickens and turkeys

  • Tibor Farkas
  • Brittney Fey
  • Edwin HargittIII
  • Mark Parcells
  • Brian Ladman
  • Maria Murgia
  • Yehia Saif
Article

DOI: 10.1007/s11262-011-0695-4

Cite this article as:
Farkas, T., Fey, B., Hargitt, E. et al. Virus Genes (2012) 44: 262. doi:10.1007/s11262-011-0695-4

Abstract

Fecal specimens, including swabs and litter extracts, collected from chickens, domestic ducks, turkeys, and Canadian geese were tested using degenerate primers targeting regions encoding for conserved amino acid motifs (YGDD and DY(T/S)(R/K/G)WDST) in calicivirus RNA-dependent RNA polymerases. Similar motifs are also present in other RNA viruses. Two fecal specimens and 18 litter extracts collected from chickens and turkeys yielded RT-PCR products. BLAST search and phylogenetic analysis revealed that all amplicons represented picornaviruses that clustered into two major groups. Four chicken and one turkey samples yielded 250 bp amplicons with 84–91% nucleotide identity to the recently described turkey hepatitis viruses, while 280 and 283 bp amplicons obtained from 11 chicken and 4 turkey samples represented novel picornaviruses with the closest nucleotide identity to kobuviruses (54–61%) and turdiviruses (47–54%). Analysis of 2.2–3.2 kb extended genome sequences including the partial P2 (2C) and complete P3 (3A, 3B (VPg), 3Cpro, and 3Dpol) regions of selected strains indicated that viruses yielding the 280/283 bp amplicons represent a putative new genus of Picornaviridae. The 3′-non-translated region (NTR) of the turkey hepatitis-like viruses described in this study was significantly longer (641–654 nt) than that of any of the other piconaviruses and included a putative short open reading frame (ORF). In summary, we report the molecular detection of novel picornaviruses that appear to be endemic in both chickens and turkeys.

Keywords

Picornavirus Chicken Turkey Turkey hepatitis 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tibor Farkas
    • 1
    • 2
  • Brittney Fey
    • 1
  • Edwin HargittIII
    • 1
  • Mark Parcells
    • 3
  • Brian Ladman
    • 3
  • Maria Murgia
    • 4
  • Yehia Saif
    • 4
  1. 1.Division of Infectious DiseasesCincinnati Children’s Hospital Medical CenterCincinnatiUSA
  2. 2.University of Cincinnati College of MedicineCincinnatiUSA
  3. 3.University of Delaware, College of Agriculture and Natural ResourcesNewarkUSA
  4. 4.Food Animal Health Research Program, Ohio Agricultural Research and Development CenterThe Ohio State UniversityWoosterUSA

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