Virus Genes

, Volume 41, Issue 1, pp 47–58

Rapid differentiation of vaccine strain and Chinese field strains of transmissible gastroenteritis virus by restriction fragment length polymorphism of the N gene

  • Chengbao Wang
  • Jianfei Chen
  • Hongyan Shi
  • Hua-Ji Qiu
  • Fei Xue
  • Shengwang Liu
  • Changming Liu
  • Yuanmao Zhu
  • Fernando Almazán
  • Luis Enjuanes
  • Li Feng
Article

DOI: 10.1007/s11262-010-0481-8

Cite this article as:
Wang, C., Chen, J., Shi, H. et al. Virus Genes (2010) 41: 47. doi:10.1007/s11262-010-0481-8

Abstract

A strain of transmissible gastroenteritis virus (TGEV), designated H16, was isolated in PK-15 cells and passaged serially to level 165. Vaccines based on passages 155–165 in cell cultures are available commercially as vaccines for the prevention and control of infections with TGEV in China. Nucleoprotein (N) sequences of the virus at passages 155 and 165 were aligned and compared using a computer software program. The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiation of the vaccine strain from the other TGEVs was investigated. The RFLP analysis identified a change in the cleavage sites of AclI at passages 155 and 165. This RFLP pattern of the N gene differentiated the Chinese vaccine strain from its parental strain, the 11 TGEVs studied and the other reported TGEVs in the GenBank. Using phylogenetic analysis, the Chinese TGEVs were divided into three groups (G1, G2, and G3). The G3 Chinese TGEVs possessed several specific nucleotides and amino acids that were not found in the G1 and G2 Chinese TGEVs or the other reference TGEVs. Analysis of the phylogenetic trees revealed that the G3 TGEVs represent a separate group that is distinct from the non-Chinese TGEVs and from Chinese TGEVs isolated previously. These findings suggest that Chinese strains of TGEV are evolving continuously.

Keywords

Transmissible gastroenteritis virus Restriction fragment length polymorphism N gene RT-PCR 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Chengbao Wang
    • 1
  • Jianfei Chen
    • 1
  • Hongyan Shi
    • 1
  • Hua-Ji Qiu
    • 1
  • Fei Xue
    • 1
  • Shengwang Liu
    • 1
  • Changming Liu
    • 1
  • Yuanmao Zhu
    • 1
  • Fernando Almazán
    • 2
  • Luis Enjuanes
    • 2
  • Li Feng
    • 1
  1. 1.Division of Swine Infectious Diseases, National Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research Institute of the Chinese Academy of Agricultural SciencesHarbinPeople’s Republic of China
  2. 2.Department of Molecular and Cell Biology, Centro Nacional de BiotecnologiaCampus Universidad AutonomaCantoblanco (Madrid)Spain

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