Article

Virus Genes

, 37:250

First online:

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

  • Max CiarletAffiliated withVaccine & Biologics – Clinical Research, Merck Research Laboratories Email author 
  • , Christian HoffmannAffiliated withDepartment of Microbiology, University of Pennsylvania
  • , Eleonora LorussoAffiliated withDipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari
  • , Rafael BaselgaAffiliated withExopol
  • , Maria Assunta CafieroAffiliated withIstituto Zooprofilattico di Puglia e Basilicata
  • , Krisztián BányaiAffiliated withVeterinary Medical Research Institute, Hungarian Academy of Sciences
  • , Jelle MatthijnssensAffiliated withDepartment of Microbiology & Immunology, Rega Institute for Medical Research, University of Leuven
  • , Viviana ParreñoAffiliated withVirology Institute, CICV y A, National Institute of Agriculture Technology (INTA)
  • , Simona de GraziaAffiliated withDipartimento di Igiene e Microbiologia, Università di Palermo
    • , Canio BuonavogliaAffiliated withDipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari
    • , Vito MartellaAffiliated withDipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari

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Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95–98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

Keywords

Rotavirus Gastroenteritis Genome Genotype Genetic diversity Reassortment Lamb VP4 VP6 VP7 NSP4 NSP5