Virus Genes

, Volume 35, Issue 2, pp 243–249

Complete genomes and phylogenetic positions of bovine papillomavirus type 8 and a variant type from a European bison

  • Yoshimi Tomita
  • Ivan Literak
  • Tomoko Ogawa
  • Zhongri Jin
  • Hiroshi Shirasawa
Original Article

DOI: 10.1007/s11262-006-0055-y

Cite this article as:
Tomita, Y., Literak, I., Ogawa, T. et al. Virus Genes (2007) 35: 243. doi:10.1007/s11262-006-0055-y

Abstract

The DNA of bovine papillomavirus (BPV) type 8 was extracted from papillomas on cattle kept in Japan, and DNA of bovine papillomavirus BPV-8-EB was extracted from a European bison (Bison bonasus) born in Italy and released into the wild in Slovakia. The DNA genomes of these BPVs were amplified using multiply primed rolling circle amplification and polymerase chain reaction, then characterized by direct sequencing method. The BPV-8 and BPV-8-EB genomes consisted of 7,791 base pairs (bp) and 7,773 bp, respectively (GenBank accession numbers DQ098913 and DQ098917). The nucleotide sequence similarity of these BPVs indicated that BPV-8-EB was a variant of BPV-8. In the genome of BPV-8-EB, one nucleotide substitution was found in the E2 and E5 open reading frame (ORF) and upstream regulatory region (URR), and a short deletion and addition were found in the URR. The high similarity of sequences between the BPV-8 to BPV-5 in total genome (70%) and L1 ORF (75%) as well as a phylogenetic analysis were the bases for classifying BPV-8 in the genus Epsilon papillomavirus. The BPV-8 E6 and E7 ORFs/proteins also showed some characteristic features of genus Epsilon papillomavirus. However, BPV-8 contained E4 ORF, which was not found in BPV-5. In addition, the secondary structure of E5 proteins of BPV-5 and BPV-8 suggested that these proteins may have cell-transforming ability.

Keywords

Bovine papillomavirusNew typeBPV-8BPV-8-EBClassificationE5 ORF

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Yoshimi Tomita
    • 1
  • Ivan Literak
    • 2
  • Tomoko Ogawa
    • 3
  • Zhongri Jin
    • 1
  • Hiroshi Shirasawa
    • 1
  1. 1.Department of Molecular Virology, Graduate School of MedicineChiba UniversityChibaJapan
  2. 2.Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and EcologyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  3. 3.Division of VirologyChiba Prefectural Institute of Public HealthChibaJapan