Veterinary Research Communications

, Volume 34, Issue 2, pp 119–132

Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanted with CpG oligonucleotides

  • Purushottam Kaushik
  • Dhirendra K. Singh
  • S. Vinoth Kumar
  • Ashok K. Tiwari
  • Gunjan Shukla
  • Shanker Dayal
  • Pallav Chaudhuri
Original Article

DOI: 10.1007/s11259-009-9337-x

Cite this article as:
Kaushik, P., Singh, D.K., Kumar, S.V. et al. Vet Res Commun (2010) 34: 119. doi:10.1007/s11259-009-9337-x

Abstract

Brucella abortus, a gram negative, facultative intracellular pathogen causes brucellosis in many animal species and humans. Although live, attenuated vaccines are available against this infection, they suffer from certain limitations. Therefore, the development of an effective subunit vaccine against brucellosis is an area of intense research. The outer membrane proteins (OMPs) of Brucella species have been extensively studied for its immunogenicity and protective ability. We have investigated the potential of CpG ODN to enhance the immunogenicity and protective efficacy of recombinant 28 kDa outer membrane protein (rOMP28) of Brucella melitensis. The study demonstrated vigorous immunoglobulin G (IgG) response of OMP28. The administration of rOMP28 with CpG caused increased cell mediated immune response in terms of induced IgG2a, T-cell proliferation and up-regulation of type I cytokine expression. In contrast, the free antigen suppressed the interferon gamma (type I cytokine) production on in-vitro stimulation of spleenocytes. The result indicates the role of OMP28 in the down regulation of IFN-γ production. Moreover, the B. abortus S-19 vaccinated mice showed highest production of IL-4 and IFN-γ. The protective ability of the antigen was evaluated by systemic bacterial clearance after challenging the mouse with B. abortus 544 pathogen. The level of protection was significant in rOMP28+CpG treated mice but was lower than the required level. The results of the present study indicate that rOMP28 could be an immunogen capable of inducing both humoral and cellular immune response. The humoral response was biased towards Th1 type when it was co-administered with CpG.

Keywords

BrucellaOMP28VaccineProtectionCpG oligonucleotide

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Purushottam Kaushik
    • 4
  • Dhirendra K. Singh
    • 1
  • S. Vinoth Kumar
    • 2
  • Ashok K. Tiwari
    • 2
  • Gunjan Shukla
    • 2
  • Shanker Dayal
    • 3
  • Pallav Chaudhuri
    • 2
  1. 1.Division of Veterinary Public HealthIndian Veterinary Research InstituteBareillyIndia
  2. 2.Division of BiotechnologyIndian Veterinary Research InstituteBareillyIndia
  3. 3.Division of Animal Genetics and BreedingBihar Veterinary CollegePatna, BiharIndia
  4. 4.Division of Veterinary Public HealthBihar Veterinary CollegePatna, BiharIndia