Veterinary Research Communications

, 33:811

Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor

  • Silvia Colleoni
  • Emanuela Bottani
  • Irene Tessaro
  • Gaetano Mari
  • Barbara Merlo
  • Noemi Romagnoli
  • Alessandro Spadari
  • Cesare Galli
  • Giovanna Lazzari
Original Article

DOI: 10.1007/s11259-009-9229-0

Cite this article as:
Colleoni, S., Bottani, E., Tessaro, I. et al. Vet Res Commun (2009) 33: 811. doi:10.1007/s11259-009-9229-0

Abstract

Mesenchymal stem cells (MSC) are increasingly used as therapeutical aid for the orthopaedic injuries in the horse. MSC populate different tissues but the most commonly used for clinical purposes are isolated from bone marrow or adipose tissue. The first objective of this study was to investigate if the donor animal, the tissue of origin and the technique of isolation could influence the number of MSC available for transplantation after a short-term expansion. The second aim was to devise a culture system capable of increasing MSC lifespan and we tested the effect of basic fibroblast growth factor (bFGF). Results indicate that MSC can be efficiently isolated from both sources and supplementation of bFGF enhances proliferation rate maintaining differentiation potential. In addition, this study shows that collection, expansion and storage of frozen MSC can be performed for later therapeutic use.

Keywords

Mesenchymal stem cellsEquineOrthopaedic injuriesBone marrowAdipose tissue

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Silvia Colleoni
    • 1
  • Emanuela Bottani
    • 1
  • Irene Tessaro
    • 1
  • Gaetano Mari
    • 2
  • Barbara Merlo
    • 2
  • Noemi Romagnoli
    • 2
  • Alessandro Spadari
    • 2
  • Cesare Galli
    • 1
    • 2
  • Giovanna Lazzari
    • 1
  1. 1.Laboratorio di Tecnologie della RiproduzioneCremonaItaly
  2. 2.Dipartimento Clinico VeterinarioUniversità di BolognaOzzano EmilaItaly
  3. 3.Sezione di Anatomia e Istologia Veterinaria, Dipartimento di Scienze Animali, Facoltà di Medicina VeterinariaUniversità degli Studi di MilanoMilanoItaly
  4. 4.Istituto Nazionale Neurologico C.BestaMilanoItaly