Phylogenetic background of attaching and effacing Escherichia coli isolates from animals

Short Communication

DOI: 10.1007/s11259-008-9042-1

Cite this article as:
Tramuta, C., Robino, P. & Nebbia, P. Vet Res Commun (2008) 32: 433. doi:10.1007/s11259-008-9042-1

Abstract

Detection and distribution of eae gene in forty-four attaching and effacing Escherichia coli (AEEC) strains of animal origin were investigated. Association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. Phylogenetic analysis showed that 31 EHEC/eae+ STEC strains belong to groups A, B1 and E, 13 EPEC strains segregated in B1 and B2. Moreover, group A possessed the eae γ2/θ type, group B1 the eae β1, eae κ, eae ζ, and eae ɛ types, group B2 the eae α1, eae α2 and eae ι types, while the group E possessed the eae γ1 type. The presence of numerous eae-types show that EPEC and EHEC/eae+ STEC tested have a high genetic homology within each phylogenetic group.

Keywords

Escherichia coli Intimin Phylogenetic analysis Animals 

Abbreviations

A/E

attaching and effacing

AEEC

attaching and effacing E. coli

DAEC

diffusely adhering E. coli

EAF

EPEC Adherence Factor

EHEC

enterohaemorrhagic E. coli

EPEC

enterophathogenic E. coli

ExPEC

extraintestinal pathogenic E. coli

LEE

locus of enterocyte effacement

PCR

polymerase chain reaction

RFLP

restriction fragment length polymorphism

STEC

Shiga toxin producing E. coli

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Dipartimento di Produzioni Animali, Epidemiologia ed EcologiaUniversità degli Studi di Torino, Facoltà di Medicina VeterinariaGrugliasco (TO)Italy