Veterinary Research Communications

, Volume 30, Issue 6, pp 637–643

Diagnosis of Canine Leishmaniasis in the Endemic Area of Belo Horizonte, Minas Gerais, Brazil by Parasite, Antibody and DNA Detection Assays

Authors

  • E. S. da Silva
    • Laboratório de LeishmaniosesCentro de Pesquisas René Rachou-Fiocruz
    • Centro de Pós-graduação e Pesquisas/Fundação Educacional de DivinópolisUniversidade do Estado de Minas Gerais
  • W. F. van der Meide
    • KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute) KIT Biomedical Research
  • G. J. Schoone
    • KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute) KIT Biomedical Research
  • C. M. F. Gontijo
    • Laboratório de LeishmaniosesCentro de Pesquisas René Rachou-Fiocruz
    • KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute) KIT Biomedical Research
  • R. P. Brazil
    • Laboratório de LeishmaniosesCentro de Pesquisas René Rachou-Fiocruz
Article

DOI: 10.1007/s11259-006-3324-2

Cite this article as:
Silva, E.S.d., Meide, W.F.v.d., Schoone, G.J. et al. Vet Res Commun (2006) 30: 637. doi:10.1007/s11259-006-3324-2

Abstract

Canine leishmaniasis caused by Leishmania chagasi (L. infantum) is found throughout the South American continent, including Brazil, and dogs are considered to be the main reservoir host for this parasite. To support the implementation of a diagnostic protocol for surveillance of the disease in the region of Belo Horizonte (Minas Gerais, Brazil) we have compared the sensitivity and specificity of two serological tests, indirect immunofluorescent antibody test (IFAT) and direct agglutination test (DAT), with the combination of direct microscopy–culture–PCR as the gold standard, using samples obtained from 103 dogs in the city of Belo Horizonte, Minas Gerais. The currently used standard serodiagnostic test, IFAT, had a sensitivity of 100% and its specificity was 74% compared to the gold standard of the study. The sensitivity and specificity of the DAT were 100% and 91%, respectively. On the basis of this study it is recommended to change from the IFAT to DAT for the serodiagnosis of canine leishmaniasis because of the superior specificity of the test combined with its user-friendliness.

Keywords

diagnosisdogleishmaniasis

Abbreviations

CanL

canine leishmaniasis

DAT

direct agglutination test

IFAT

indirect immunofluorescent antibody test

MRBH

Metropolitan Region of Belo Horizonte

NNN

Novy–McNeal–Nicolle

PCR

polymerase chain reaction

Copyright information

© Springer Science + Business Media, Inc. 2006