Veterinary Research Communications

, Volume 30, Issue 3, pp 307–317

Feline Immunodeficiency Virus and Puma Lentivirus in Florida panthers (Puma concolor coryi): Epidemiology and Diagnostic Issues

  • D. L. Miller
  • S. K. Taylor
  • D. S. Rotstein
  • M. B. Pough
  • M. C. Barr
  • C. A. Baldwin
  • M. Cunningham
  • M. Roelke
  • D. Ingram
Virology

DOI: 10.1007/s11259-006-3167-x

Cite this article as:
Miller, D.L., Taylor, S.K., Rotstein, D.S. et al. Vet Res Commun (2006) 30: 307. doi:10.1007/s11259-006-3167-x

Abstract

This study documents the seroprevalence of feline immunodeficiency virus (FIV) and puma lentivirus (PLV) in free-ranging and captive Florida panthers (Puma concolor coryi) (n = 51) and translocated Texas cougars (P. concolor stanleyana) (n = 10) from 1985 to 1998. The sera were tested for anti-FIV antibodies by enzyme-linked immunosorbent assay (ELISA) and Western blot tests. The ELISAs were read kinetically (KELA) and the sera were retrospectively examined by PLV peptide ELISA. Eleven panthers and one cougar were positive by KELA; 4 panthers and 4 cougars were equivocal; 35 panthers and 5 cougars were negative; and 1 panther had no data. Seven of the 11 KELA-positive panthers were also positive by Western blot tests and all but one were positive by PLV peptide ELISA. Ten KELA-negative and Western blot-negative cats, were positive by PLV peptide ELISA. KELA results varied within cats from one sample period to the next, but PLV peptide ELISA results were consistent. Territorial sympatry and mating behaviour, noted from radiotelemetry location data on the cats, may have contributed to viral transmission between seropositive animals. These findings suggest that Florida panthers and the introduced Texas cougars have been exposed to FIV and/or PLV.

Keywords

cougarfeline immunodeficiency virusFIVFlorida pantherKELApuma lentivirusPLVpeptide ELISA

Abbreviations

CBC

complete blood count

ELISA

enzyme-linked immunosorbent assay

FIV

feline immunodeficiency virus

FP

Florida panther

KELA

kinetic ELISA

PLV

puma lentivirus

RBC

red blood cell count

TX

Texas cougar

WBC

white blood cell count

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • D. L. Miller
    • 1
  • S. K. Taylor
    • 2
    • 7
  • D. S. Rotstein
    • 3
  • M. B. Pough
    • 4
  • M. C. Barr
    • 5
  • C. A. Baldwin
    • 1
  • M. Cunningham
    • 2
  • M. Roelke
    • 6
  • D. Ingram
    • 1
  1. 1.Veterinary Diagnostic and Investigational Laboratory, College of Veterinary MedicineUniversity of GeorgiaTifton
  2. 2.Florida Fish and Wildlife Conservation CommissionGainesville
  3. 3.Department of Pathobiology, College of Veterinary MedicineUniversity of TennesseeKnoxville
  4. 4.Developmental Serology Diagnostic Laboratory, College of Veterinary MedicineCornell UniversityIthaca
  5. 5.Western University of Health SciencesCollege of Veterinary MedicinePamona
  6. 6.Laboratory of Genomic DiversityNational Cancer InstituteFrederickUSA
  7. 7.National Center for Environmental Assessment–Washington, DC DivisionEnvironmental Protection AgencyResearch Triangle ParkUSA