International Urology and Nephrology

, Volume 46, Issue 4, pp 731–736

Comparison of erythropoietin and sildenafil protective role against ischemia/reperfusion injury of the testis in adult rats

Authors

  • Nick Zavras
    • Third Department of Surgery, Attikon University HospitalNational and Kapodistrian University of Athens, Medical School
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
  • Stratigoula Sakellariou
    • First Department of PathologyNational and Kapodistrian University of Athens, Medical School
  • Christos Damaskos
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
  • Evangelos Roupakas
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
  • Eleni Tsagkari
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
  • Eleftherios Spartalis
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
  • Konstantinos Velaoras
    • Department of Pediatric SurgeryPenteli General Children’s Hospital
  • Ismene A. Dontas
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
  • Theodore Karatzas
    • Laboratory of Experimental Surgery and Surgical Research “N.S. Christeas”National and Kapodistrian University of Athens, Medical School
    • Second Department of Propedeutic Surgery, “Laiko” General HospitalNational and Kapodistrian University of Athens, Medical School
Urology - Original Paper

DOI: 10.1007/s11255-013-0569-x

Cite this article as:
Zavras, N., Kostakis, I.D., Sakellariou, S. et al. Int Urol Nephrol (2014) 46: 731. doi:10.1007/s11255-013-0569-x

Abstract

Purpose

Tissue damage in testicular torsion/detorsion is caused not only by the ischemia, but also by the ischemia/reperfusion injury after detorsion. Erythropoietin and sildenafil are considered to protect against ischemia/reperfusion injury. Here, we studied and compared their actions in testicular torsion/detorsion in adult rats.

Methods

Twenty-two adult male Wistar Albino rats were divided into four groups. Rats in group A (n = 5) were sham operated. Rats in group B (n = 5), group C (n = 6) and group D (n = 6) underwent torsion of the right testis and detorsion after 90 min. No pharmaceutical intervention was performed in group B. Erythropoietin (1,000 IU/kg) and sildenafil (0.7 mg/kg) were injected intraperitoneally in groups C and D, respectively, after 60 min of torsion. All animals were killed 24 h after detorsion, and their right testis was extracted, placed into 10 % formalin solution and sent for histopathological examination. The histological changes in the testes were scored according to the four-point grading system proposed by Cosentino et al.

Results

All rats in group A had normal testicular architecture (grade 1). The untreated group B had a mean grade of 3.81 (range 3.65–4). The treated groups C (mean grade 3.24; range 3.05–3.45) and D (2.69, range 2.4–2.9) presented statistically significant better results (lower grades) compared with the untreated group B. Group D had significantly better results (lower grades) than group C.

Conclusions

The intraperitoneal injection of erythropoietin and sildenafil protects against ischemia/reperfusion injury after testicular torsion and detorsion. Sildenafil may have a stronger action than erythropoietin at the doses used in this study.

Keywords

Testicular torsionIschemia/reperfusion injuryErythropoietinSildenafilRats

Copyright information

© Springer Science+Business Media Dordrecht 2013