International Urology and Nephrology

, Volume 44, Issue 1, pp 111–117

Promoter methylation of H-cadherin is a potential biomarker in patients with bladder transitional cell carcinoma

  • Ying-Li Lin
  • Xiao-Qiang Liu
  • Wen-Ping Li
  • Guang Sun
  • Chun-Ting Zhang
Urology – Original Paper

DOI: 10.1007/s11255-011-9961-6

Cite this article as:
Lin, YL., Liu, XQ., Li, WP. et al. Int Urol Nephrol (2012) 44: 111. doi:10.1007/s11255-011-9961-6

Abstract

Objectives

H-cadherin, functions as a tumor suppressor, is frequently silenced by promoter methylation in human cancers. The aim of this study was to evaluate the feasibility of using H-cadherin methylation in tumor tissues as a potential biomarker in patients with bladder transitional cell carcinoma (TCC).

Materials and methods

We examined the methylation status of H-cadherin in 133 primary bladder TCC samples and 43 normal bladder epithelial tissues using methylation-specific polymerase chain reaction (MSP) and then analyzed the associations between H-cadherin methylation and clinicopathologic features as well as patients’ outcome.

Results

H-cadherin methylation was detected in 47 (35.3%) bladder TCC samples, but not found in controls (P = 0.0000). Moreover, H-cadherin methylation was significantly associated with advanced stage (P = 0.0006), high grade (P = 0.0165), larger tumor size (P = 0.0225), tumor recurrence (P = 0.0106), and poor prognosis (P = 0.0000). In addition, multivariate analysis indicated that H-cadherin methylation is independently associated with poor outcome and had a relative risk of death of 3.832 (P = 0.0071, 95% confidence interval: 1.443–10.176).

Conclusions

The results suggest that H-cadherin methylation may be used as a potential biomarker for the malignancy of bladder TCC and as an independent prognostic biomarker in patients with bladder TCC.

Keywords

Bladder cancer Methylation H-cadherin Transitional cell carcinoma Biomarker 

Copyright information

© Springer Science+Business Media, B.V. 2011

Authors and Affiliations

  • Ying-Li Lin
    • 1
    • 2
  • Xiao-Qiang Liu
    • 1
    • 2
  • Wen-Ping Li
    • 3
  • Guang Sun
    • 2
  • Chun-Ting Zhang
    • 1
    • 2
  1. 1.Department of UrologyThe Second Hospital of Tianjin Medical UniversityTianjinChina
  2. 2.Tianjin Institute of UrologyTianjinChina
  3. 3.Department of UrologyThe Third Hospital of Hebei Medical UniversityShijiazhuang, HebeiChina

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