International Urology and Nephrology

, Volume 42, Issue 2, pp 325–330

Evaluation of routine application of P504S, 34βE12 and p63 immunostaining on 250 prostate needle biopsy specimens

  • Kitty Pavlakis
  • Konstantinos Stravodimos
  • Theodoros Kapetanakis
  • Alkiviadis Gregorakis
  • Sofia Athanassiadou
  • Olympia Tzaida
  • Constantinos Constantinides
Urology - Original Paper

DOI: 10.1007/s11255-009-9622-1

Cite this article as:
Pavlakis, K., Stravodimos, K., Kapetanakis, T. et al. Int Urol Nephrol (2010) 42: 325. doi:10.1007/s11255-009-9622-1

Abstract

Background

We aimed to determine whether a standard P504S, 34βE12 and p63 immunostaining of prostate core needle biopsy specimens can optimize diagnostic accuracy of conventional staining methods.

Materials and methods

The staining properties of all three antibodies were evaluated on 250 prostate biopsies formerly diagnosed as benign.

Results

Lack of basal cell staining in more than three glands for 34βE12 and p63 occurred in 41 (27.5%) and 9 (6%) respective cases from the General Hospital pool. Respective figures from the Uropathology department specimens were 18 (18%) for 34βE12 and 8 (8%) for p63. With the aid of P504S positivity, a case of prostate cancer as well as another of atypical small acinar proliferation was discovered.

Conclusion

Despite its potential for important aid in accurate diagnosis, standard application of immunohistochemistry in prostate biopsy is not justified and should be reserved for equivocal cases where conventional pathology fails to be conclusive.

Keywords

ImmunostainingProstate cancerP504S34βE12p63

Copyright information

© Springer Science+Business Media, B.V. 2009

Authors and Affiliations

  • Kitty Pavlakis
    • 1
  • Konstantinos Stravodimos
    • 2
  • Theodoros Kapetanakis
    • 2
    • 4
  • Alkiviadis Gregorakis
    • 3
  • Sofia Athanassiadou
    • 1
  • Olympia Tzaida
    • 1
  • Constantinos Constantinides
    • 2
  1. 1.Pathology Department, “ATTIKON” University HospitalUniversity of Athens Medical SchoolAthensGreece
  2. 2.1st Urology Department, “Laikon” General HospitalUniversity of Athens Medical SchoolAthensGreece
  3. 3.2nd Urology Department, “ATTIKON” General HospitalUniversity of Athens Medical SchoolAthensGreece
  4. 4.Resident in UrologyAthensGreece