International Urology and Nephrology

, Volume 41, Issue 1, pp 85–91

The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study

  • Burhan Köseoğlu
  • Engin Yılmaz
  • Kadir Ceylan
  • Ersan Uzun
  • İrfan Bayram
  • Fatih Hızlı
Urology - Original Paper

DOI: 10.1007/s11255-008-9418-8

Cite this article as:
Köseoğlu, B., Yılmaz, E., Ceylan, K. et al. Int Urol Nephrol (2009) 41: 85. doi:10.1007/s11255-008-9418-8



The aim of the present study was to evaluate the effects of erythropoietin (EPO) on the histopathology of testes after unilateral testicular torsion and detorsion.

Materials and methods

Twenty-five male Sprague–Dawley rats weighing 120 g were used in this study. The rats were randomly divided into three groups, a sham group consisting of five rats and the other two groups consisting of ten rats. In group 1 (sham group), right orchiectomy with no additional intervention was performed. In group 2 (T/D group), torsion was created by rotating the testis 720° in a clockwise direction for 4 h. After a 4-h torsion period, the right testis was detorted and replaced into the scrotum for 4 h. After the torsion, 0.5 cc 0.9% NaCl solution was injected once and three times in a week (total 12 doses). In group 3 (T/D + erythropoietin; EPO group), the same surgical procedure was done as in group 1, but EPO 1,000 IU/kg was injected just before the detorsion and three times in a week. At the end of each procedure, bilateral orchiectomies were performed for the histopathological examinations in all groups.


We examined the testes weight, vascularization of the region between the seminiferous tubules, percentage of necrotic seminipherous tubules, and maturation of spermatogenesis in terms of necrosis, sertoli cells, maturation arrest of spermatogenesis, hypospermatogenesis, and normal spermatogenesis of torsioned testis tissues with and without EPO treatment. Extremely significant differences in testicular weight were observed in group 1 compared to groups 2 and 3 (P < 0.001).


Administration of EPO significantly influenced the rescue of testicular function by preserving the intact seminiferous tubular morphology, lowering the percentage of necrotic seminipherous tubules, and significantly reducing histological damage (P < 0.05).


Testicular torsion Erythropoietin Atrophy Necrosis Histological damage 

Copyright information

© Springer Science+Business Media, B.V. 2008

Authors and Affiliations

  • Burhan Köseoğlu
    • 1
  • Engin Yılmaz
    • 2
  • Kadir Ceylan
    • 5
  • Ersan Uzun
    • 3
  • İrfan Bayram
    • 4
  • Fatih Hızlı
    • 6
  1. 1.Department of Pediatric SurgeryFatih University School of MedicineAnkaraTurkey
  2. 2.Department of Pediatric SurgeryBakirköy Education and Research HospitalÍstanbulTurkey
  3. 3.Department of Pediatric SurgeryYüzüncü Yıl University School of MedicineVanTurkey
  4. 4.Department of PathologyYüzüncü Yıl University School of MedicineVanTurkey
  5. 5.Department of UrologyYüzüncü Yıl University School of MedicineVanTurkey
  6. 6.Department of UrologyOncology Education and Research HospitalAnkaraTurkey