Abstract
This study was conducted to assess the seroprevalence and clinical relevance of Corynebacterium pseudotuberculosis, which is the causative agent of caseous lymphadenitis (CLA), in native Korean goats (Capra hircus coreanae). A total of 466 native Korean goats from 40 herds (11 to 12 samples per herd) were randomly selected throughout the nation and evaluated by direct palpation, bacterial isolation, ELISA, and PCR. In serological examinations, 267 (57.3 %) of the goats tested were positive against C. pseudotuberculosis. When seroprevalence was analyzed according to age, region, and season, statistically significant differences were observed in relation to all three parameters (P < 0.05). For clinical examination, the superficial lymph nodes of all goats were palpated to diagnose CLA. Pus samples taken from superficial abscesses were used for bacterial isolation. Among the 466 goats tested, 34 (7.3 %) were presumptively diagnosed with CLA, and C. pseudotuberculosis was isolated from 24 goats (70.6 % of goats with CLA lesions) whose infections were confirmed by PCR. Considering the high seroprevalence and bacterial isolation rate from most of the superficial CLA lesions, it is suspected that many internal CLA lesions exist in this goat population. These results suggest that C. pseudotuberculosis infection is widespread in native Korean goats, and appropriate control programs need to be established.
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References
Ashfaq, M.K. and Campbell, S.G., 1980. Experimentally induced caseous lymphadenitis in goats, American Journal of Veterinary Research, 41, 1789–1792
Augustine, J.L. and Renshaw, H.W., 1986. Survival of Corynebacterium pseudotuberculosis in axenic purulent exudate on common barnyard fomites, American Journal of Veterinary Research, 47, 713–715
Baird, G.J. and Malone, F.E., 2010. Control of caseous lymphadenitis in six sheep flocks using clinical examination and regular ELISA testing, Veterinary Record, 166, 358–362
Brown, C. and Olander, H., 1987. Caseous lymphadenitis of goats and sheep: a review, Veterinary Bulletin, 57, 1–12
Dercksen, D., Ter Laak, E. and Schreuder, B., 1996. Eradication programme for caseous lymphadenitis in goats in the Netherlands. Veterinary Record, 138, 237
Dorella, F.A., Pacheco, L.G.C., Oliveira, S.C., Miyoshi, A. and Azevedo, V., 2006. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence, Veterinary Research, 37, 201–218
Ellis, J.A., Hawk, D.A., Holler, L.D., Mills, K.W. and Pratt, D.L., 1990. Differential antibody responses to Corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis, Journal of the American Veterinary Medical Association, 196, 1609–1613
Gezon, H.M., Bither, H.D., Hanson, L.A. and Thompson, J.K., 1991. Epizootic of external and internal abscesses in a large goat herd over a 16-year period, Journal of American Veterinary Medical Association, 198, 257–263
KOSIS. Korean Statistical Information Service. http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1AG501&conn_path=I2. Accessed 15 August 2010.
Menzies, P., Muckle, C., Hwang, Y. and Songer, J., 1994. Evaluation of an enzyme-linked immunosorbent assay using an Escherichia coli recombinant phospholipase D antigen for the diagnosis of Corynebacterium pseudotuberculosis infection, Small Ruminant Research, 13, 193–198
Pacheco, L.G., Pena, R.R., Castro, T.L., Dorella, F.A., Bahia, R.C., Carminati, R., Frota, M.N., Oliveira, S.C., Meyer, R., Alves, F.S., Miyoshi, A. and Azevedo, V., 2007. Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples, Journal of Medical Microbiology, 56, 480–486
Pepin, M., Marly, J. and Pardon, P., 1987. Corynebacterium pseudotuberculosis infection in sheep and the complement fixation test for paratuberculosis, Veterinary Record, 120, 236
Seyffert, N., Guimarães, A., Pacheco, L., Portela, R., Bastos, B., Dorella, F., Heinemann, M., Lage, A., Gouveia, A. and Meyer, R., 2010. High seroprevalence of caseous lymphadenitis in Brazilian goat herds revealed by Corynebacterium pseudotuberculosis secreted proteins-based ELISA, Research in Veterinary Science, 88, 50–55
Shin, D., Song, Y., Byun, J., Kim, H., Kim, H., Woo, G., Lee, O. and Jung, B., 2010. Caseous lymphadenitis by Corynebacterium pseudotuberculosis in a Saanen dairy goat (Capra hircus aegagrus), Korean Journal of Veterinary Research, 50, 25–28
Skalka, B., Literak, I., Michalik, I. and Skřivánek, M., 1998. Corynebacterium pseudotuberculosis infection in goats in the Czech Republic, Journal of Veterinary Medicine, Series B, 45, 31–35
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This work was supported financially by the Animal and Plant Quarantine Agency (B-AD21-2010-11-03), Ministry of Agriculture, Food and Rural Affairs, Korea.
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The authors have no financial, personal, or organizational conflict with respect to the work reported in this manuscript.
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Jung, B.Y., Lee, SH., Kim, HY. et al. Serology and clinical relevance of Corynebacterium pseudotuberculosis in native Korean goats (Capra hircus coreanae). Trop Anim Health Prod 47, 657–661 (2015). https://doi.org/10.1007/s11250-015-0773-z
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DOI: https://doi.org/10.1007/s11250-015-0773-z