Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR
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Brucellosis is endemic in bovines in Pakistan. The Brucella species and biovars involved, however, are unknown. The objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. The seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the Potohar Plateau, Pakistan. Isolation of brucellae was done on modified Farrell’s serum dextrose agar. Isolates were characterized by conventional biotyping methods, while molecular typing was done by genus (B4/B5) and species-specific (Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis) polymerase chain reaction (PCR). A total of 30 isolates were recovered from milk (n = 5), aborted fetuses (n = 13), and vaginal swabs (n = 12). Most isolates were from cattle (56.7 %). All of them were identified as B. abortus biovar 1 based on conventional biotyping methods and genus and species-specific PCR. This preliminary study provides the first report on the prevalence of B. abortus biovar 1 in cattle and buffaloes in Pakistan.
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- Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR
Tropical Animal Health and Production
Volume 46, Issue 1 , pp 73-78
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- Springer Netherlands
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- Brucella abortus
- Biochemical tests
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- Author Affiliations
- 1. Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, 46300, Pakistan
- 2. Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Straße 96a, 07743, Jena, Germany
- 3. National Veterinary Laboratory, Park Road, Islamabad, Pakistan
- 4. University of Veterinary and Animal Sciences, Lahore, Pakistan
- 5. Ministry of National Food Security and Research, Islamabad, Pakistan