Tropical Animal Health and Production

, Volume 45, Issue 3, pp 829–836

Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon

Authors

    • Department of Animal SciencesUniversity of Dschang
    • School of Veterinary Medicine and Sciences, University of Ngaoundere
    • School of Biomedical and Biological SciencesUniversity of Plymouth
  • Ayuba Caleb Kudi
    • School of Biomedical and Biological SciencesUniversity of Plymouth
    • Department of Veterinary MedicineAhmadu Bello University
  • Graham Bradley
    • School of Biomedical and Biological SciencesUniversity of Plymouth
  • Noel H. Smith
    • Animal Health and Veterinary Laboratories Agency
  • Irene Ane-Anyangwe
    • Department of Biochemistry and Microbiology, University of Buea
  • Fidelis Cho-Ngwa
    • Department of Biochemistry and Microbiology, University of Buea
  • Vicent Pryde Kehdinga Titanji
    • Department of Biochemistry and Microbiology, University of Buea
Regular Articles

DOI: 10.1007/s11250-012-0295-x

Cite this article as:
Awah-Ndukum, J., Kudi, A.C., Bradley, G. et al. Trop Anim Health Prod (2013) 45: 829. doi:10.1007/s11250-012-0295-x

Abstract

An epidemiological study was carried out to determine the Mycobacterium bovis strains causing bovine tuberculosis (TB) in cattle in North West Cameroon. Suspected TB lesions from slaughtered cattle were cultured on Lowenstein–Jensen and Middlebrook 7 H9 media to isolate mycobacteria agents for molecular genotyping using deletion analysis and spoligotyping. PCR-based genomic deletion typing showed that 54 of 103 tubercle bacilli isolated from cattle tissue were M. bovis strains and the African 1 clonal complex was widespread in affected cattle. Spoligotyping analysis revealed a closely related group of five M. bovis strains. SB0953, the dominant spoligotype pattern, and four new patterns identified as SB2161, SB2162, SB2663 and SB2664 according to the www.Mbovis.org international spoligotype database were identified. These spoligotypes were similar to other M. bovis strains recovered from bordering regions and other parts of Africa. The findings provided useful facts on the zoonotic risks of bovine TB and overwhelming evidence of the significance of M. bovis infection to human TB in the North West Region of Cameroon. The study revealed that bovine TB was widespread in cattle destined for human consumption and also has important implications for the control of TB in animals and humans in Cameroon.

Keywords

CattleTuberculous lesionsMycobacterium bovisMolecular genotypingZoonosisNorth West Cameroon

Copyright information

© Springer Science+Business Media Dordrecht 2012