Tropical Animal Health and Production

, Volume 42, Issue 5, pp 985–993

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Authors

  • Shamik Polley
    • Animal Biotechnology CentreNational Dairy Research Institute
  • Sachinandan De
    • Animal Biotechnology CentreNational Dairy Research Institute
    • Krishi Vigyan Kendra, SKUAST-Jammu
  • Ayan Mukherjee
    • Animal Biotechnology CentreNational Dairy Research Institute
  • Vinesh P.V.
    • Animal Biotechnology CentreNational Dairy Research Institute
  • Subhasis Batabyal
    • West Bengal University of Animal and Fishery Sciences
  • Jaspreet Singh Arora
    • Animal Biotechnology CentreNational Dairy Research Institute
  • Subhransu Pan
    • West Bengal University of Animal and Fishery Sciences
  • Ashis Kumar Samanta
    • West Bengal University of Animal and Fishery Sciences
  • Tirtha Kumar Datta
    • Animal Biotechnology CentreNational Dairy Research Institute
  • Surender Lal Goswami
    • Animal Biotechnology CentreNational Dairy Research Institute
Article

DOI: 10.1007/s11250-009-9518-1

Cite this article as:
Polley, S., De, S., Brahma, B. et al. Trop Anim Health Prod (2010) 42: 985. doi:10.1007/s11250-009-9518-1

Abstract

Mutation studies in different prolific sheep breeds have shown that the transforming growth factor beta super family ligands viz. the growth differentiation factor 9 (GDF9/FecG), bone morphogenetic protein 15 (BMP15/FecX) and associated type I receptors, bone morphogenetic protein receptor (BMPR1B/FecB), are major determinant of ovulation rate and consequent increase in litter size. The Garole sheep is a highly prolific sheep breed of India. Characterization of fecundity genes in these animals could substantially improvise the breeding programme in these animals as well as other sheep breeds of the region. The present study was therefore designed with the objective of polymorphism study of fecundity genes in these prolific microsheep. A total of 11 point mutations were detected by polymerase chain reaction (PCR)-based method. A competitive technique called tetra-primer amplification refractory mutation system-PCR was adapted to type a total of ten points of two ovine fecundity genes (GDF9 and BMP15). The FecB locus of the BMPR1B gene and G1 locus of GDF9 gene were found to be polymorphic. In FecB locus, two genotypes, wild type (FecB+) and mutant (FecBB), were detected with allele frequencies of 0.39 and 0.61, respectively. At G1 locus, two genotypes, mutant (A) and wild types (G) were detected with allele frequencies of 0.18 and 0.82, respectively. This study reports Garole sheep as the fourth sheep breed after Belclare/Cambridge, Lacaune and Small-tailed Han sheep, where coexisting polymorphism has been found in two different fecundity genes (BMPRIB and GDF9 genes).

Keywords

Garole sheepProlificacyBMPR1BGDF9BMP15

Copyright information

© Springer Science+Business Media B.V. 2009