Nutritional evaluation of transgenic cottonseed in the ration of lactating dairy cows
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The effects of feeding transgenic (Bt) whole cottonseed (WCS) were studied in lactating cows. Twenty multiparous crossbred cows (Karan Swiss × Karan Fries) in early lactation were given a concentrate mixture containing 40% crushed delinted non-transgenic (non-Bt) WCS, 2 kg wheat straw and green fodder ad lib for a 15-day adaptation period. Thereafter, the cows were divided in two similar groups of 10 each on the basis of milk yield, body weight (BW) and date of calving. The non-Bt control group continued on same ration, while for the Bt group the non-Bt WCS was replaced by transgenic WCS, in a feeding trial of four weeks. The diets provided a minimum of 2 kg cottonseed/cow/d. Mean DMI/100 kg BW and milk yield of non-Bt and Bt groups was 3.48 and 3.45 kg and 11.4 and 12.0 kg/d, respectively. Intake of nutrients, digestibility, milk production and body condition score (BCS) did not differ between the groups (P > 0.05), but BW gain was higher (P < 0.05) in the Bt group than the non-Bt group, probably as a result of hoof problem in two cows of non-Bt group, which when compared excluding two animals from each group did not differ significantly (P > 0.05). Transgenic protein (Cry1C) was not detected in the weekly milk samples or in blood plasma at the end of the experiment, showing that delinted WCS containing Cry1C protein can safely be fed to lactating cows.
- Alexander, T. W., Reuter, T., Aulrich, K., Sharma, R., Okaine, E. K., Dixon, W. T., and McAllister, T. A. 2007. A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production. Animal Feed Science and Technology, 133, 31–62 CrossRef
- AOAC, 1990. Official Methods of Analysis, 15th edition, Association of Official Analytical Chemists, Arlington, VA, USA.
- Arieli, A., 1998. Whole cottonseed in dairy cattle feeding: A review. Animal Feed Science and Technology, 72, 97–110 CrossRef
- CAST (Council for Agricultural Science and Technology), 2006. Safety of meat, milk, and eggs from animals fed crops derived from modern biotechnology, Issue Paper No. 34, CAST, Ames, Iowa, USA. pp 1–8
- Castillo, R., Gallardo, M.R., Maciel, M., Giordano, J.M., Conti, G.A., Gaggiotti, M.C., Quaino, O., Gianni, C. and Hartnell, G.F., 2004. Effects of feeding rations with genetically modified whole cottonseed to lactating Holstein cows. Journal of Dairy Science, 87, 1778–1785
- Flachowsky, G., Aulrich, K., Bohme, H. and Halle, I., 2007. Studies on feeds from genetically modified plants (GMP)-contributions to nutritional and safety assessment. Animal Feed Science and Technology, 133, 2–30 CrossRef
- Goering, H. K. and Van Soest, P. J., 1967. Forage fibre analysis (Apparatus, reagents, procedures, and some application). Agriculture Handbook No. 379, ARS-USDA, Washington, DC.
- Hofte, H. and Whiteley H.R., 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews, 53(2), 242–255
- Huls, T.J., Erickson, G.E., Klopfenstein, T.J., Luebbe, M.K., Vander, K.J., Rice, D.W., Smith, B., Hinds, M., Owens, F. and Liebergesell, M., 2008. Effect of feeding DAS-59122-7 corn grain and non-transgenic corn grain to individually fed finishing steers. Professional Animal Scientist, 24, 572–577
- IDF, 1984. Recommended methods for somatic cell counting in milk. Bulletin of International Dairy Federation, 168, 4–6
- Kaneko, J.J., 1997. Clinical Biochemistry of Domestic Animals, 5th edn, Academic Press, San Diego, CA., USA.
- Kumar, R.S. and Singhal, K.K., 2004. Chemical composition and nutritional evaluation of transgenic cottonseed for ruminants. Indian Journal of Animal Sciences, 74 (5), 868–871.
- Moreira, V.R., Satter, L.D. and Harding, B., 2004.Comparison of conventional linted cottonseed and mechanically delinted cottonseed in diets for dairy cows. Journal of Dairy Science, 87, 131–138 CrossRef
- Mujahid, A., Abdullah, M., Barque, A.R. and Gilani, A.H., 2000. Nutritional value of cottonseeds and its derived products: I. Physical fractionations and proximate composition. Asian–Australasian Journal of Animal Sciences, 13, 348–355
- NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th revised edition, National Research Council, National Academy of Sciences, Washington, DC, USA.
- Prasad, S., 1994. Studies on body condition scoring and feeding management in relation to production performance of crossbred cows. Ph. D. Thesis, National Dairy Research Institute, Karnal, Haryana, India.
- Raghuramulu, N, Nair, K.M., Kalyanasundaram, S., 2003. A manual of laboratory techniques. National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India
- Ranjhan, S.K., 1998. Nutrient requirement of livestock and poultry, Indian Council of Agricultural Research (ICAR) Publication, New Delhi, India.
- Saijpaul, S., Grewal, R.S., Ahuja, C.S. and Naik, P.K., 2006. Effect of supplementation of whole linted cottonseed on the performance and blood biochemical profile of crossbred cows in summer. Animal Nutrition and Feed Technology, 6, 95–102
- Shimada, N., Murata, H. & Miyazaki, S., 2008. Safety evaluation of Bt plants. The Japan Agricultural Research Quarterly, 42, 251–259
- Singh, M. and Ludhri, R.S., 2001. Somatic cell counts in Murrah buffaloes (Bubalus bubalis) during different stages of lactation, parity and season. Asian–Australasian Journal of Animal Sciences, 14, 189–192
- Singh, M., Tiwari, D.P., Kumar, A., and Ravi Kumar, M., 2003. Effect of feeding transgenic cottonseed vis-à-vis non-transgenic cottonseed on haemato-biochemical constituents in lactating Murrah buffaloes. Asian–Australasian Journal of Animal Sciences, 16, 1732–1737
- Singhal, K.K., Kumar, R.S., Tyagi, A.K. and Rajput, Y.S., 2006. Evaluation of Bt cottonseed as protein supplement in the ration of lactating dairy cows. Indian Journal of Animal Sciences, 76, 532–537
- Smith, F.H., 1968. Estimation of free gossypol in cottonseed meal and cottonseed meats: modified method. Journal of American Oil Chemical Society, 45, 903 CrossRef
- Stein, H.H., Rice, D.W., Smith, B.L., Hinds, M.A., Sauber, T.E., Pedersen, C., Wulf, D.M. and Peters, D.N., 2009. Evaluation of corn grain with the genetically modified input trait DAS-59122-7 fed to growing-finishing pigs. Journal of Animal Science, 87, 1254–1260 CrossRef
- SYSTAT, 1997. Statistical package for social sciences Version 7.0. SPSS Inc., Chicago, IL, USA.
- Talapatra, S.K., Roy, S.C., and Sen, K.C., 1940. The analysis of mineral constituents in biological materials: Estimation of phosphorus, chlorine, calcium, manganese, sodium and potassium in foodstuffs. Indian Journal of Veterinary Science and Animal Husbandry, 10, 243–258
- Van Keulen, J. and Young, B.A., 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestion studies. Journal of Animal Science, 44, 282–287
- Nutritional evaluation of transgenic cottonseed in the ration of lactating dairy cows
Tropical Animal Health and Production
Volume 42, Issue 3 , pp 431-438
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- Bt protein
- Feed intake
- Industry Sectors
- Author Affiliations
- 1. Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, India
- 2. Animal Biochemistry Division, National Dairy Research Institute, Karnal, India
- 3. Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, India