Tropical Animal Health and Production

, Volume 39, Issue 2, pp 91–95

Concurrent infection with bovine leukaemia virus and Theileria annulata in a Friesian calf

Authors

    • Department of Veterinary Medicine, College of Agriculture and Veterinary MedicineQassim University
  • O. H. Omer
    • Department of Veterinary Medicine, College of Agriculture and Veterinary MedicineQassim University
  • O. M. Mahmoud
    • Department of Veterinary Medicine, College of Agriculture and Veterinary MedicineQassim University
  • M. E. Hashad
    • Department of Veterinary Medicine, College of Agriculture and Veterinary MedicineQassim University
Original Article

DOI: 10.1007/s11250-006-4393-5

Cite this article as:
Al-Dubaib, M., Omer, O.H., Mahmoud, O.M. et al. Trop Anim Health Prod (2007) 39: 91. doi:10.1007/s11250-006-4393-5

Abstract

Concurrent infection with bovine leukaemia virus (BLV) and Theileria annulata was diagnosed in a Friesian calf about 6 months of age at a dairy farm at the Qassim region of central Saudi Arabia. The disease ended fatally with signs of liver and heart failure. There was anorexia, pyrexia, anaemia, generalized oedema and jaundice. Haematology showed low RBC counts, PCV percentage and haemoglobin concentration and WBC counts. Lymphocyte differential was high. Examination of blood smears stained with Giemsa’s stain showed the presence of piroplasms in red blood cells. Autopsy showed enlarged lymph nodes and lymphosarcoma lesions in the omentum and the heart. There was hydroperitoneum, hydropericardium and hydrothorax. The liver was pale yellow and friable. Impression smears from sliced lymph nodes and stained with Giemsa’s stain showed presence of Koch’s blue bodies in lymphoblasts. Histopathological examination revealed fatty degeneration of hepatocytes and pleomorphic lymphoblasts and giant cells in lymph nodes. Lymphoblasts infiltrated the omentum and heart tissues. Amyloid was found around blood vessels in the liver, kidneys and lymph nodes. BVL infection was diagnosed by demonstrating antibodies against the virus in serum using agar gel immunodiffusion and was confirmed with ELISA.

Keywords

Concurrent infectionTheileriosisBovine leukaemia virus

Abbreviations

AGID

agar gel immunodiffusion test

BLV

bovine leukaemia virus

CBC

complete blood count

OD

optical density

PCV

packed cell volume

RBC

red blood cell

TMB

3,3,5,5-tetramethylbenzidine

WBC

white blood cell

Copyright information

© Springer Science + Business Media B.V. 2007