Transgenic Research

, Volume 21, Issue 3, pp 537–543

Production of cloned transgenic cow expressing omega-3 fatty acids

  • Xia Wu
  • Hongsheng Ouyang
  • Biao Duan
  • Daxin Pang
  • Li Zhang
  • Ting Yuan
  • Lian Xue
  • Daibang Ni
  • Lei Cheng
  • Shuhua Dong
  • Zhuying Wei
  • Lin Li
  • Ming Yu
  • Qing-Yuan Sun
  • Da-Yuan Chen
  • Liangxue Lai
  • Yifan Dai
  • Guang-Peng Li
Original Paper

DOI: 10.1007/s11248-011-9554-2

Cite this article as:
Wu, X., Ouyang, H., Duan, B. et al. Transgenic Res (2012) 21: 537. doi:10.1007/s11248-011-9554-2

Abstract

n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.

Keywords

N-3 fatty acid desaturase genemfat-1Transgenic cattleNuclear transfer

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Xia Wu
    • 1
  • Hongsheng Ouyang
    • 2
  • Biao Duan
    • 1
  • Daxin Pang
    • 2
  • Li Zhang
    • 1
  • Ting Yuan
    • 2
  • Lian Xue
    • 1
  • Daibang Ni
    • 2
  • Lei Cheng
    • 1
  • Shuhua Dong
    • 1
  • Zhuying Wei
    • 1
  • Lin Li
    • 3
  • Ming Yu
    • 3
  • Qing-Yuan Sun
    • 4
  • Da-Yuan Chen
    • 4
  • Liangxue Lai
    • 2
  • Yifan Dai
    • 3
  • Guang-Peng Li
    • 1
  1. 1.The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia UniversityHohhotPeople’s Republic of China
  2. 2.College of Animal Sciences and Veterinary Technology, Jilin UniversityChangchunPeople’s Republic of China
  3. 3.State Key Laboratory of Reproductive Medicine, Center of Metabolic Disease Research, Nanjing Medical UniversityNanjingPeople’s Republic of China
  4. 4.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesBeijingPeople’s Republic of China