Original Paper

Transgenic Research

, Volume 21, Issue 3, pp 537-543

First online:

Production of cloned transgenic cow expressing omega-3 fatty acids

  • Xia WuAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
  • , Hongsheng OuyangAffiliated withCollege of Animal Sciences and Veterinary Technology, Jilin University
  • , Biao DuanAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
  • , Daxin PangAffiliated withCollege of Animal Sciences and Veterinary Technology, Jilin University
  • , Li ZhangAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
  • , Ting YuanAffiliated withCollege of Animal Sciences and Veterinary Technology, Jilin University
  • , Lian XueAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
  • , Daibang NiAffiliated withCollege of Animal Sciences and Veterinary Technology, Jilin University
  • , Lei ChengAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
    • , Shuhua DongAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
    • , Zhuying WeiAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University
    • , Lin LiAffiliated withState Key Laboratory of Reproductive Medicine, Center of Metabolic Disease Research, Nanjing Medical University
    • , Ming YuAffiliated withState Key Laboratory of Reproductive Medicine, Center of Metabolic Disease Research, Nanjing Medical University
    • , Qing-Yuan SunAffiliated withState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
    • , Da-Yuan ChenAffiliated withState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
    • , Liangxue LaiAffiliated withCollege of Animal Sciences and Veterinary Technology, Jilin University
    • , Yifan DaiAffiliated withState Key Laboratory of Reproductive Medicine, Center of Metabolic Disease Research, Nanjing Medical University
    • , Guang-Peng LiAffiliated withThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University Email author 

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Abstract

n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.

Keywords

N-3 fatty acid desaturase gene mfat-1 Transgenic cattle Nuclear transfer