Transgenic Research

, Volume 21, Issue 1, pp 139–147

Transgenic production of arachidonic acid in oilseeds

  • James R. Petrie
  • Pushkar Shrestha
  • Srinivas Belide
  • Maged P. Mansour
  • Qing Liu
  • James Horne
  • Peter D. Nichols
  • Surinder P. Singh
Original Paper

DOI: 10.1007/s11248-011-9517-7

Cite this article as:
Petrie, J.R., Shrestha, P., Belide, S. et al. Transgenic Res (2012) 21: 139. doi:10.1007/s11248-011-9517-7

Abstract

We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We also demonstrate that the arachidonic acid is naturally enriched at the sn2 position in triacylglycerol. This is the first report of ARA production by the Δ9-elongase pathway in an oilseed.

Keywords

Arachidonic acid LC-PUFA Delta-9 elongase Delta-8 desaturase Brassica napus 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • James R. Petrie
    • 1
  • Pushkar Shrestha
    • 1
  • Srinivas Belide
    • 1
    • 2
  • Maged P. Mansour
    • 3
  • Qing Liu
    • 1
  • James Horne
    • 4
  • Peter D. Nichols
    • 3
  • Surinder P. Singh
    • 1
  1. 1.Food Futures National Research FlagshipCSIRO Plant IndustryCanberraAustralia
  2. 2.Department of BiotechnologySreenidhi Institute of Science and TechnologyHyderabadIndia
  3. 3.Food Futures National Research FlagshipCSIRO Marine and Atmospheric ResearchHobartAustralia
  4. 4.Central Science LaboratoryUniversity of TasmaniaHobartAustralia

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