Transgenic Research

, Volume 20, Issue 4, pp 773–786

Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens

  • Xudong Ye
  • Edward J. Williams
  • Junjiang Shen
  • Susan Johnson
  • Brenda Lowe
  • Sharon Radke
  • Steve Strickland
  • James A. Esser
  • Michael W. Petersen
  • Larry A. Gilbertson
Original Paper

DOI: 10.1007/s11248-010-9458-6

Cite this article as:
Ye, X., Williams, E.J., Shen, J. et al. Transgenic Res (2011) 20: 773. doi:10.1007/s11248-010-9458-6

Abstract

Single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. We demonstrate that binary vectors that use a replication origin derived from the Ri plasmid of Agrobacterium rhizogenes (oriRi) increase the frequency of single copy, backbone-free transgenic plants in Agrobacterium tumefaciens mediated transformation of soybean, canola, and corn, compared to RK2-derived binary vectors (RK2 oriV). In large scale soybean transformation experiments, the frequency of single copy, backbone-free transgenic plants was nearly doubled in two versions of the oriRi vectors compared to the RK2 oriV control vector. In canola transformation experiments, the oriRi vector produced more single copy, backbone-free transgenic plants than did the RK2 oriV vector. In corn transformation experiments, the frequency of single copy backbone-free transgenic plants was also significantly increased when using the oriRi vector, although the transformation frequency dropped. These results, derived from transformation experiments using three crops, indicate the advantage of oriRi vectors over RK2 oriV binary vectors for the production of single copy, backbone-free transgenic plants using Agrobacterium-mediated transformation.

Keywords

Agrobacterium-mediated transformationTransgenic plantsBinary vectorCopy numberSingle copy backbone-free transgenic plantSoybean transformationCanola transformationCorn transformation

Supplementary material

11248_2010_9458_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Xudong Ye
    • 1
  • Edward J. Williams
    • 1
  • Junjiang Shen
    • 1
  • Susan Johnson
    • 4
  • Brenda Lowe
    • 2
  • Sharon Radke
    • 3
  • Steve Strickland
    • 3
  • James A. Esser
    • 1
  • Michael W. Petersen
    • 1
  • Larry A. Gilbertson
    • 4
  1. 1.Agracetus Campus, Monsanto CompanyMiddletonUSA
  2. 2.Mystic Research, Monsanto CompanyMysticUSA
  3. 3.Calgene Campus, Monsanto CompanyDavisUSA
  4. 4.Monsanto CompanySt. LouisUSA