Transgenic Research

, Volume 20, Issue 3, pp 701–707

Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR

  • Tommaso Barbi
  • Pascal M. W. Drake
  • Matthew Drever
  • Craig J. van Dolleweerd
  • Andrew R. Porter
  • Julian K-C. Ma
Brief Communication

DOI: 10.1007/s11248-010-9431-4

Cite this article as:
Barbi, T., Drake, P.M.W., Drever, M. et al. Transgenic Res (2011) 20: 701. doi:10.1007/s11248-010-9431-4

Abstract

In this paper we describe the engineering and regeneration of transgenic tobacco plants expressing a recombinant plasma membrane-retained antibody specific to microcystin-LR (MC-LR), the environmental toxin pollutant produced by cyanobacteria. The antibody was created by a genetic fusion of the antigen binding regions of the microcystin-specific single chain antibody, 3A8, with the constant regions from the murine IgG1κ, Guy’s 13, including a membrane retention sequence at the C-terminal end of the antibody heavy chain. The antibody produced in the leaves was shown to be functional by binding to MC-LR in an ELISA with antibody yields in transgenic plant leaves reaching a maximum of 1.2 μg g−1 leaf f.wt (0.005% total soluble protein). Antibody-MC-LR complexes formed in leaves after addition of MC-LR to hydroponic medium around the roots of transgenic plant cultures.

Keywords

Microcystin-LRTransgenic plantsAntibodiesPhytoremediation

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Tommaso Barbi
    • 1
  • Pascal M. W. Drake
    • 1
  • Matthew Drever
    • 2
  • Craig J. van Dolleweerd
    • 1
  • Andrew R. Porter
    • 2
  • Julian K-C. Ma
    • 1
  1. 1.Molecular Immunology Unit, Centre for Infection, Division of Clinical SciencesSt. George’s University of LondonLondonUK
  2. 2.Department of Molecular and Cell Biology, Foresterhill School of Medical SciencesUniversity of AberdeenAberdeenUK