Transgenic Research

, Volume 20, Issue 1, pp 177–181

Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content

  • Shaista Naqvi
  • Gemma Farré
  • Changfu Zhu
  • Gerhard Sandmann
  • Teresa Capell
  • Paul Christou
Brief Communication

DOI: 10.1007/s11248-010-9393-6

Cite this article as:
Naqvi, S., Farré, G., Zhu, C. et al. Transgenic Res (2011) 20: 177. doi:10.1007/s11248-010-9393-6

Abstract

The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to ρ-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constitutively expressed in corn to further characterize the pathway and increase the kernel tocopherol content. Transgenic kernels contained up to 3 times as much γ-tocopherol as their wild type counterparts whereas other tocopherol isomers remained undetectable. Biofortification by metabolic engineering offers a sustainable alternative to vitamin E supplementation for the improvement of human health.

Keywords

Vitamin ETocopherolsMetabolic engineeringTransgenic maize

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Shaista Naqvi
    • 1
  • Gemma Farré
    • 1
  • Changfu Zhu
    • 1
  • Gerhard Sandmann
    • 2
  • Teresa Capell
    • 1
  • Paul Christou
    • 1
    • 3
  1. 1.Departament de Producció Vegetal i Ciència ForestalUniversitat de LleidaLleidaSpain
  2. 2.Biosynthesis Group, Molecular BiosciencesJohann Wolfgang Goethe UniversitätFrankfurtGermany
  3. 3.Institucio Catalana de Recerca i Estudis AvancatsBarcelonaSpain