Transgenic Research

, Volume 19, Issue 3, pp 511–518

Comparative effects of dietary administered transgenic and conventional papaya on selected intestinal parameters in rat models

  • Melissa Powell
  • Andrew O. Wheatley
  • Felix Omoruyi
  • Helen N. Asemota
  • Nadia P. Williams
  • Paula F. Tennant
Brief Communication

DOI: 10.1007/s11248-009-9317-5

Cite this article as:
Powell, M., Wheatley, A.O., Omoruyi, F. et al. Transgenic Res (2010) 19: 511. doi:10.1007/s11248-009-9317-5

Abstract

Transformation of agricultural crops with novel genes has significantly advanced disease-resistance breeding, including virus resistance through the expression of virus sequences. In this study, the effects of long-term, repeated exposure to transgenic papayas carrying the coat protein gene of Papaya ringspot virus and conventional non-transgenic papaya on the histology and selected biochemical parameters of the intestinal tract were compared. For 3 months, male and female Wistar rats received diets containing transgenic or non-transgenic papaya at twice the equivalent of the average daily consumption of fresh papayas. Gross and macroscopic appearance of intestinal tissues, as well as stomach tissues, was comparable (P < 0.05) as were total intestinal bacterial counts and activities of β-glucuronidase. Activities of disaccharidases were not affected, neither were those of amylase (P < 0.05). Although significant differences were noted in the activity of Ca2+ and Na+/K+ ATPase brush border enzymes, no morphological alteration in the integrity of the intestinal mucosa was found. Overall, negligible effects on feed intake, body weight, and fecal output were observed (P < 0.05). Taken together, long-term exposure to diets formulated with transgenic papaya did not result in biologically important unintended effects.

Keywords

AmylaseATPaseCarica papayaDisaccharidaseHistopathology

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Melissa Powell
    • 1
  • Andrew O. Wheatley
    • 1
    • 2
  • Felix Omoruyi
    • 1
  • Helen N. Asemota
    • 1
    • 2
  • Nadia P. Williams
    • 3
  • Paula F. Tennant
    • 2
    • 4
  1. 1.Department of Basic Medical Sciences, Biochemistry SectionUniversity of the West IndiesMonaJamaica
  2. 2.Biotechnology CentreUniversity of the West IndiesMonaJamaica
  3. 3.Department of PathologyUniversity of the West IndiesMonaJamaica
  4. 4.Department of Life SciencesUniversity of the West IndiesMonaJamaica