Original Paper

Transgenic Research

, Volume 18, Issue 4, pp 513-527

First online:

Induction of focal epithelial hyperplasia in tongue of young bk6-E6/E7 HPV16 transgenic mice

  • Rodolfo Ocadiz-DelgadoAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico
  • , Alberto Marroquin-ChaviraAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico
  • , Ruth Hernandez-MoteAffiliated withDepartment of Pathology, Hospital Infantil de Mexico “Federico Gómez”, Mexico
  • , Concepción ValenciaAffiliated withInstitute of Biotechnology-UNAM Mexico
  • , M. Eugenia Manjarrez-ZavalaAffiliated withInstituto Nacional de Enfermedades Respiratorias (INER), Mexico
  • , Luis CovarrubiasAffiliated withInstitute of Biotechnology-UNAM Mexico
  • , Patricio GariglioAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico Email author 

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Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly expressed and cellular proliferation was increased in the middle tongue epithelia of transgenic mice, and that in the same region young (27 weeks old) Tg[bK6-E6/E7] mice spontaneously developed histological alterations, mainly focal epithelial hyperplasia (FEH).


Tongue HPV Focal epithelial hyperplasia Proliferation Apoptosis