Transgenic Research

, Volume 18, Issue 4, pp 513–527

Induction of focal epithelial hyperplasia in tongue of young bk6-E6/E7 HPV16 transgenic mice


  • Rodolfo Ocadiz-Delgado
    • Department of Genetics & Molecular BiologyCINVESTAV-IPN Mexico
  • Alberto Marroquin-Chavira
    • Department of Genetics & Molecular BiologyCINVESTAV-IPN Mexico
  • Ruth Hernandez-Mote
    • Department of PathologyHospital Infantil de Mexico “Federico Gómez”, Mexico
  • Concepción Valencia
    • Institute of Biotechnology-UNAM Mexico
  • M. Eugenia Manjarrez-Zavala
    • Instituto Nacional de Enfermedades Respiratorias (INER), Mexico
  • Luis Covarrubias
    • Institute of Biotechnology-UNAM Mexico
    • Department of Genetics & Molecular BiologyCINVESTAV-IPN Mexico
Original Paper

DOI: 10.1007/s11248-009-9243-6

Cite this article as:
Ocadiz-Delgado, R., Marroquin-Chavira, A., Hernandez-Mote, R. et al. Transgenic Res (2009) 18: 513. doi:10.1007/s11248-009-9243-6


Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly expressed and cellular proliferation was increased in the middle tongue epithelia of transgenic mice, and that in the same region young (27 weeks old) Tg[bK6-E6/E7] mice spontaneously developed histological alterations, mainly focal epithelial hyperplasia (FEH).


TongueHPVFocal epithelial hyperplasiaProliferationApoptosis

Copyright information

© Springer Science+Business Media B.V. 2009