Original Paper

Transgenic Research

, Volume 18, Issue 4, pp 513-527

First online:

Induction of focal epithelial hyperplasia in tongue of young bk6-E6/E7 HPV16 transgenic mice

  • Rodolfo Ocadiz-DelgadoAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico
  • , Alberto Marroquin-ChaviraAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico
  • , Ruth Hernandez-MoteAffiliated withDepartment of Pathology, Hospital Infantil de Mexico “Federico Gómez”, Mexico
  • , Concepción ValenciaAffiliated withInstitute of Biotechnology-UNAM Mexico
  • , M. Eugenia Manjarrez-ZavalaAffiliated withInstituto Nacional de Enfermedades Respiratorias (INER), Mexico
  • , Luis CovarrubiasAffiliated withInstitute of Biotechnology-UNAM Mexico
  • , Patricio GariglioAffiliated withDepartment of Genetics & Molecular Biology, CINVESTAV-IPN Mexico Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly expressed and cellular proliferation was increased in the middle tongue epithelia of transgenic mice, and that in the same region young (27 weeks old) Tg[bK6-E6/E7] mice spontaneously developed histological alterations, mainly focal epithelial hyperplasia (FEH).

Keywords

Tongue HPV Focal epithelial hyperplasia Proliferation Apoptosis