Transgenic Research

, Volume 17, Issue 6, pp 1155–1162

A novel hairless mouse model on an atopic dermatitis-prone genetic background generated by receptor-mediated transgenesis

Authors

  • Toyoyuki Takada
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
    • Transdisciplinary Research Integration CenterResearch Organization of Information and Systems
  • Hiroshi Shitara
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
  • Kunie Matsuoka
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
    • PROBRAIN, Promotion of Basic Research Activities for Innovative Biosciences
  • Erika Kojima
    • Department of Immune RegulationTokyo Medical and Dental University
  • Rie Ishii
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
  • Yoshiaki Kikkawa
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
    • Department of Bioproduction, Faculty of BioindustryTokyo University of Agriculture
  • Choji Taya
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
  • Hajime Karasuyama
    • Department of Immune RegulationTokyo Medical and Dental University
  • Kenji Kohno
    • PROBRAIN, Promotion of Basic Research Activities for Innovative Biosciences
    • The Graduate School of Biological SciencesNara Institute of Science and Technology
    • Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical Science (Rinshoken)
    • PROBRAIN, Promotion of Basic Research Activities for Innovative Biosciences
Brief Communication

DOI: 10.1007/s11248-008-9203-6

Cite this article as:
Takada, T., Shitara, H., Matsuoka, K. et al. Transgenic Res (2008) 17: 1155. doi:10.1007/s11248-008-9203-6

Abstract

Current mouse models for atopic dermatitis (AD) have a serious drawback, being the existence of dense hair on the body. Thus, a hairless animal model on an AD-prone genetic background will be a powerful tool to investigate the basis of and therapy for this complex disease. We applied the Toxin Receptor-mediated Cell Knockout (TRECK) method to generate a hairless transgenic (Tg) mice on the NC/Nga background, an AD-prone inbred strain. A minigene with the mouse Keratin71 (Krt71) promoter and human diphtheria toxin receptor, which intrinsically functions as the heparin-binding EGF-like growth factor, was introduced into the pronucleus of NC/Nga oocytes. Unexpectedly NCN24, one NC/Nga Tg line, showed a dominant hairless phenotype without diphtheria toxin administration. Furthermore, the atopic dermatitis-like predisposition and IgE elevation was observed in both NCN24 and the NC/Nga wildtype strain. NCN24 mice, which we have newly developed, will be useful to assess drugs for AD therapy, being able to monitor skin inflammation without shaving.

Keywords

Receptor-mediated transgenesisNC/Nga inbred strainHairless mouse modelAtopy

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2008