Transgenic Research

, 18:17

Genetic transformation of the sugar beet plastome

  • Francesca De Marchis
  • Yongxin Wang
  • Piergiorgio Stevanato
  • Sergio Arcioni
  • Michele Bellucci
Original Paper

DOI: 10.1007/s11248-008-9193-4

Cite this article as:
De Marchis, F., Wang, Y., Stevanato, P. et al. Transgenic Res (2009) 18: 17. doi:10.1007/s11248-008-9193-4

Abstract

It is very important for the application of chloroplast engineering to extend the range of species in which this technology can be achieved. Here, we describe the development of a chloroplast transformation system for the sugar beet (Beta vulgaris L. ssp. vulgaris, Sugar Beet Group) by biolistic bombardment of leaf petioles. Homoplasmic plastid-transformed plants of breeding line Z025 were obtained. Transformation was achieved using a vector that targets genes to the rrn16/rps12 intergenic region of the sugar beet plastome, employing the aadA gene as a selectable marker against spectinomycin and the gfp gene for visual screening of plastid transformants. gfp gene transcription and protein expression were shown in transplastomic plants. Detection of GFP in Comassie blue-stained gels suggested high GFP levels. Microscopy revealed GFP fluorescence within the chloroplasts. Our results demonstrate the feasibility of engineering the sugar beet chloroplast genome; this technology provides new opportunities for the genetic improvement of this crop and for social acceptance of genetically modified sugar beet plants.

Keywords

Aminoglicoside 3′-adenylyltransferase (aadABiolistic apparatus Chloroplast DNA transformation Green fluorescent protein Spectinomycin Shoot regeneration 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Francesca De Marchis
    • 1
  • Yongxin Wang
    • 1
    • 2
  • Piergiorgio Stevanato
    • 3
  • Sergio Arcioni
    • 1
  • Michele Bellucci
    • 1
  1. 1.Institute of Plant Genetics – Research Division of PerugiaItalian National Research Council (CNR)PerugiaItaly
  2. 2.Institute of Animal Science, CAASBeijingChina
  3. 3.Dip. to Biotechnologie AgrarieUniversità degli Studi di PadovaLegnaro (PD)Italy

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